Mouse MPO ELISA Kit

SKU
EA100598
Mouse MPO ELISA Kit
  $755.00
3 Weeks*
Specifications
Specifications
Product Data
Species Mouse
Assay Type Sandwich ELISA kit of Quantitative Detection for Mouse MPO/Myeloperoxidase
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 156 pg/ml-10,000 pg/ml
Sample Type Cell culture supernates, Cell lysates, tissue homogenates, serum and plasma(heparin, EDTA).
Sample Volume 100 ul
Specificity This kit is used for quantitative detection of mouse MPO
Sensitivity <10pg/ml
Reactivity Mouse
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Interference No significant interference observed with available related molecules.
Components
  • 96-well plate precoated with anti-mouse Mpo antibody | 1
  • Lyophilized recombinant mouse Mpo standard | 10ng/tube x 2
  • Biotinylated anti-mouse Mpo antibody | 130ul (dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) | 130ul(dilution 1:100)
  • Sample diluent buffer | 30ml
  • Antibody diluent buffer | 12ml
  • ABC diluent buffer | 12ml
  • TMB color developing agent | 10ml
  • TMB stop solution | 10ml
  • Plate Sealers | 4
  • Wash Buffer (25x) | 20 ml
Background Myeloperoxidase(MPO) is a mammalian phagocyte hemoprotein thought to primarily mediate host defense reactions. It is abundantly expressed in neutrophils and secreted during their activation. Myeloperoxidase is part of the host defense system of human polymorphonuclear leukocytes, responsible for microbicidal activity against a wide range of organisms. It is located in the nucleus as well as in the cytoplasm. Intranuclear MPO may help to protect DNA against damage resulting from oxygen radicals produced during myeloid cell maturation and function.
Gene Symbol Mpo
Locus ID 17523
Shipping Blue Ice
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Documents
Resources

file-alt Citations

Antioxidant Activity of Yogurt Fermented at Low Temperature and Its Anti-inflammatory Effect on DSS-induced Colitis in Mice
Yoon, Ahn, Jhoo et al
Food Sci Anim Resour (2019) 39 (1), 162-176 DOI: 10.5851/kosfa.2019.e13
AI Summary: OriGene's Myeloperoxidase (MPO) kit was used in this study to measure MPO levels in colon tissue, helping assess the anti-inflammatory effects of yogurt fermented at different temperatures in mice with DSS-induced colitis.
Snippet: Color development solution and stop solution were added to each well and the plate was read at 450 nm wavelength. Myeloperoxidase (MPO) analysis. Contents of MPO in colon tissue were measured using an MPO kit (Origene, USA)
FigureFigureFigure
Showing 1-1 of 1 papers.
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