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Human THBS1/TSP1 ELISA Kit

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SKU
EA100563
Human THBS1/TSP1 ELISA Kit
  $755.00
3 Weeks*
Specifications
Specifications
Product Data
Species Human
Assay Type Sandwich ELISA kit of Quantitative Detection for Human THBS1/TSP1
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 1.56 ng/ml-100 ng/ml
Sample Type Cell culture supernates, serum and plasma(heparin, EDTA).
Sample Volume 100 ul
Specificity This kit is used for quantitative detection of human THBS1
Sensitivity <50pg/ml
Reactivity Human
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Interference No significant interference observed with available related molecules.
Components
  • 96-well plate precoated with anti- human THBS1/TSP1 antibody 1
  • Lyophilized recombinant human THBS1/TSP1 standard 100 ng/tubex2
  • Biotinylated anti- human THBS1/TSP1 antibody 100 ul (dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 100 ul (dilution 1:100)
  • Sample diluent buffer 30 ml
  • Antibody diluent buffer 12 ml
  • ABC diluent buffer 12 ml
  • TMB color developing agent 10 ml
  • TMB stop solution 10 ml
  • Wash Buffer (25x) 20 ml
  • Plate Sealers 4 Piece
Background Thrombospondin 1, also known as THBS1, is a protein that in humans in encoded by the THBS1 gene. Thrombospondin 1 is a subunit of a disulfide-linked homotrimeric protein. This protein is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. By in situ hybridization, the THBS1 gene was mapped to human 15q15 and the cognate gene to mouse chromosome 2(region F) and was localized to 15q11-qter by Southern analysis of human-rodent somatic cell hybrids. Thrombospondin I is a multimodular secreted protein that associates with the extracellular matrix and possesses a variety of biologic functions, including a potent antiangiogenic activity. Other thrombospondin genes include thrombospondins II, III, and IV.
Gene Symbol THBS1
Locus ID 7057
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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