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Mouse KIM1 ELISA Kit

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SKU
EA100555
Mouse KIM1 ELISA Kit
  $625.00
3 Weeks*
Specifications
Specifications
Product Data
Species Mouse
Assay Type Sandwich ELISA kit of Quantitative Detection for Mouse KIM1
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 31.2pg/ml-2000pg/ml
Sample Type Cell culture supernates, tissue homogenates, serum, plasma(heparin, EDTA) and urine.
Sample Volume 100 ul
Specificity This kit is used for quantitative detection of Mouse KIM1
Sensitivity <2pg/ml
Reactivity Mouse
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Interference No significant interference observed with available related molecules.
Components
  • 96-well plate precoated with anti- mouse KIM1 antibody 1
  • Lyophilized recombinant mouse KIM1 standard 10ng/tubex2
  • Biotinylated anti- mouse KIM1 antibody 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100)
  • Sample diluent buffer 30ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
Background KIM1(TIM-1), also known as Hepatitis A virus cellular receptor 1, is a protein that in Mouses is encoded by the HAVCR1 gene.123 Infection of canine osteogenic sarcoma cells expressing HAVCR1 with HAV led Feigelstock et al.(1998) to conclude that the protein is indeed a receptor for the virus. Immunofluorescence microscopy demonstrated internalization of HAV by dog cells expressing HAVCR1. Using a monoclonal antibody to mouse Tim1, Umetsu et al.(2005) showed that Tim1 was expressed after activation of naive T cells and on T cells differentiated in Th2-polarizing conditions. By homology of synteny with the mouse Tim1 gene and database analysis, McIntire et al.(2001) mapped the HAVCR1 gene to 5q33.2. The standard used in this kit is recombinant mouse KIM-1, constituting from Y22-T212 amino acids, and 191amino acids with the molecular mass of 21.8KDa.
Gene Symbol Havcr1
Locus ID 171283
Shipping Blue Ice
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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