Rat TrkA ELISA Kit

CAT#: EA100535

Rat TrkA ELISA Kit

Datasheet
SDS

USD 705.00

3 Weeks*

Size
    • 1 x 96 wells

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Specifications

Product Data
Assay Type Sandwich ELISA kit of Quantitative Detection for Rat trkA
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 156pg/ml-10000pg/ml
Sample Type Cell culture supernates, Cell lysates and tissue homogenates.
Sample Volume 100 ul
Specificity This kit is used for quantitative detection of rat TRKA
Sensitivity <10pg/ml
Reactivities Rat
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Interference No significant interference observed with available related molecules.
Components
  • 96-well plate precoated with anti- rat TrkA antibody 1
  • Lyophilized recombinant rat TrkA standard 10ng/tubex2
  • Biotinylated anti- rat TrkA antibody 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100)
  • Sample diluent buffer 30ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
Background Tropomyosin receptor kinase A(TrkA) are efficacious in attenuating skeletal pain.1 TrkA mutants were able to activate signaling cascades and were even more efficient in promoting neurite outgrowth than the wild-type receptor.2 TrkA is part of a sub-family of protein kinases which includes TrkB and TrkC. Also, there are other neurotrophic factors structurally related to NGF: BDNF(for Brain-Derived Neurotrophic Factor), NT-3(for Neurotrophin-3) and NT-4(for Neurotrophin-4). While TrkA mediates the effects of NGF, TrkB is bound and activated by BDNF, NT-4, and NT-3. Further, TrkC binds and is activated by NT-3.3 TrkA receptor was found in keratoconus-affected corneas, along with an increased level of repressor isoform of Sp3 transcription factor.4 The standard product used in this kit is extracellular part(A33-P418) of recombinant rat TRKA, consisting of 386 amino acids with the molecular weight of 69KDa.
Gene Symbol Ntrk1
Gene ID 59109

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