Human TFPI ELISA Kit

SKU
EA100530
Human TFPI ELISA Kit
  $755.00
3 Weeks*
Specifications
Specifications
Product Data
Species Human
Assay Type Sandwich ELISA kit of Quantitative Detection for Human TFPI
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 31.2pg/ml-2000pg/ml
Sample Type Cell culture supernates, serum, plasma(heparin, EDTA) and urine.
Sample Volume 100 ul
Specificity This kit is used for quantitative detection of human TFPI
Sensitivity <10pg/ml
Reactivity Human
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Interference No significant interference observed with available related molecules.
Components
  • 96-well plate precoated with anti- human TFPI antibody 1
  • Lyophilized recombinant human TFPI standard 10ng/tubex2
  • Biotinylated anti- human TFPI antibody 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100)
  • Sample diluent buffer 30ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
Background TFPI(Tissue factor pathway inhibitor),also known as EPI, LACI or TFPI1, is a single-chain polypeptide which can reversibly inhibit Factor Xa(Xa) and Thrombin(Factor IIa). The gene for TFPI is located on chromosome 2q31-q32.1, and has nine exons which span 70 kb. TFPI consists of a highly negatively-charged amino-terminus, three tandemly-linked Kunitz domains, and a highly positively-charged carboxy-terminus.TFPI contributes significantly to the inhibition of Xa in vivo, despite being present at concentrations of only 2.5 nM.
Gene Symbol TFPI
Locus ID 7035
Shipping Blue Ice
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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