CAT#: EA100487

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USD 534.00

3 Weeks

    • 1 x 96 wells

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Product Data
Description Human HBEGF ELISA Kit
Size 1 x 96 wells
Assay Type Sandwich ELISA kit of Quantitative Detection for Human HBEGF
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 15.6pg/ml-1000pg/ml
Sample Type Cell culture supernates, serum and plasma(heparin, EDTA).
Sample Volume 100 ul
Specificity This kit is used for quantitative detection of human HBEGF
Sensitivity <10pg/ml
Reactivity Human
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Interference No significant interference observed with available related molecules.
  • 96-well plate precoated with anti- human HBEGF antibody 1
  • Lyophilized recombinant human HBEGF standard 10ng/tubex2
  • Biotinylated anti- human HBEGF antibody 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100)
  • Sample diluent buffer 30ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
Background HBEGF(Heparin-binding EGF-like growth factor), also known as HEGFL or DTR, is a member of the EGF family of proteins that in humans is encoded by the HBEGF gene. The HBEGF gene is assigned to chromosome 5, thus confirming the assignment of the gene on the basis of its role in relation to diphtheria toxin susceptibility. HB-EGF is an 87 amino acid glycoprotein which displays highly regulated gene expression. It has been shown to play a role in wound healing, cardiac hypertrophy and heart development and function. HB-EGF binding and activation of EGF receptors plays a critical role during cardiac valve tissue development and the maintenance of normal heart function in adults.
Gene Symbol HBEGF
Gene ID 1839
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