Human Galectin-3/LGALS3 ELISA Kit

CAT#: EA100482

Human Galectin-3/LGALS3 ELISA Kit

Specifications

Product Data

• Assay Type: Sandwich ELISA kit of Quantitative Detection for Human Galectin-3/LGALS3
• Assay Length: 3.5 hours incubations; 1 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 156pg/ml-10,000pg/ml
• Sample Type: Cell culture supernates, serum and plasma(heparin, EDTA).
• Sample Volume: 100 ul
• Specificity: This kit is used for quantitative detection of human Galectin-3
• Sensitivity: <10pg/ml
• Reactivities: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Interference: No significant interference observed with available related molecules.
• Components:
  • 96-well plate precoated with anti- human Galectin-3/LGALS3 antibody 1
  • Lyophilized recombinant human Galectin-3/LGALS3 standard 10ng/tubex2
  • Biotinylated anti- human Galectin-3/LGALS3 antibody 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100)
  • Sample diluent buffer 30ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
• Background: Galectin-3(GAL3), also known as LGALS3, MAC2 or GALBP, is a member of the lectin family, of which 14 mammalian galectins have been identified. Galectin-3 is encoded by a single gene, LGALS3, located on chromosome 14, locus q21–q22. It is expressed in the nucleus, cytoplasm,mitochondrion, cell surface, and extracellular space. Studies have also shown that the expression of galectin-3 is implicated in a variety of processes associated with heart failure, including myofibroblast proliferation, fibrogenesis, tissue repair, inflammation, and Ventricular remodeling. Galectin-3 is expressed in various tissues and organs, but is significantly absent in normal hepatocytes.
• Gene Symbol: LGALS3
• Gene ID: 3958