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Mouse Decorin ELISA Kit

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SKU
EA100475
Mouse Decorin ELISA Kit
  $755.00
3 Weeks*
Specifications
Specifications
Product Data
Species Mouse
Assay Type Sandwich ELISA kit of Quantitative Detection for Mouse Decorin
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 31.2pg/ml-2000pg/ml
Sample Type Cell culture supernates, serum and plasma(heparin, EDTA).
Sample Volume 100 ul
Specificity This kit is used for quantitative detection of mouse Decorin
Sensitivity <10pg/ml
Reactivity Mouse
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Interference No significant interference observed with available related molecules.
Components
  • 96-well plate precoated with anti- mouse Decorin antibody 1
  • Lyophilized recombinant mouse Decorin standard 10ng/tubex2
  • Biotinylated anti- mouse Decorin antibody 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100)
  • Sample diluent buffer 30ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
Background Decorin is a proteoglycan on average 90 - 140 kilodaltons(kD) in size.It belongs to the small leucine-rich proteoglycan(SLRP) family and consists of a protein core containing leucine repeats with a glycosaminoglycan(GAG) chain consisting of either chondroitin sulfate(CS) or dermatan sulfate(DS).Using a genomic clone as the labeled probe and in situ hybridization of human metaphase chromosomes, decorin gene was mapped to the discrete region of human chromosome 12q23. Decorin is capable of suppressing the growth of various tumor cell lines when expressed ectopically.
Gene Symbol Dcn
Locus ID 13179
Shipping Blue Ice
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Documents
Resources

file-alt Citations

An essential role for decorin in bladder cancer invasiveness
El Behi, Krumeich, Lodillinsky et al
EMBO Mol Med (2013)
Cited by 53
AI Summary: OriGene’s pCMV6-Entry plasmid containing mouse Decorin cDNA (NM_007833) was used to achieve decorin overexpression in MB49 cells, enabling the study of decorin’s role in promoting bladder cancer invasiveness.
Snippet: Decorin overexpression in MB49 cells was achieved using the pCMV6‐ Entry plasmid containing cDNA of mouse Decorin (NM_007833) purchased from Origene (Rockville, MD, USA)
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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