Human CD40/TNFRSF5 ELISA Kit

CAT#: EA100448

Human CD40/TNFRSF5 ELISA Kit


 Product Datasheet for 'EA100448'

USD 535.00


Availability*
3 Weeks

Size
    • 96-Well

Product images

Specifications

Product Data
Description Human CD40/TNFRSF5 ELISA Kit
Size 96-well
Format 8×12 divisible strips
Assay Type Sandwich ELISA kit of Quantitative Detection for Human CD40
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 15.6pg/ml-1000pg/ml
Sample Type Cell culture supernates, serum, plasma(heparin, EDTA), saliva, urine and human milk.
Sample Volume 100 ul
Specificity This kit is used for quantitative detection of human CD40
Sensitivity <1pg/ml
Reactivity Human
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Interference No significant interference observed with available related molecules.
Components
  • 96-well plate precoated with anti- human CD40/TNFRSF5 antibody 1
  • Lyophilized recombinant human CD40/TNFRSF5 standard 10ng/tubex2
  • Biotinylated anti- human CD40/TNFRSF5 antibody 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100)
  • Sample diluent buffer 30ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
Background CD40 is a cell surface receptor that is expressed on the surface of all mature B cells, most mature B-cell malignancies, and some early B-cell acute lymphocytic leukemias, but is not expressed on plasma cells. The CD40 gene is localized to the long arm of chromosome 20, bands q12-q13.2. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation.3
Gene Symbol CD40
Gene ID 958

Documents

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