Mouse Cystatin C ELISA Kit

CAT#: EA100433

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Mouse Cystatin C ELISA Kit



USD 567.00


Availability*
3 Weeks

Size
    • 1 x 96 wells

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Specifications

Product Data
Assay Type Sandwich ELISA kit of Quantitative Detection for Mouse Cystatin C
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 312pg/ml-20,000pg/ml
Sample Type Cell culture supernates, serum, plasma(heparin, EDTA) and urine.
Sample Volume 100 ul
Specificity This kit is used for quantitative detection of mouse Cystatin C
Sensitivity <10pg/ml
Reactivities Mouse
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Interference No significant interference observed with available related molecules.
Components
  • 96-well plate precoated with anti- mouse Cystatin C antibody 1
  • Lyophilized recombinant mouse Cystatin C standard 20ng/tubex2
  • Biotinylated anti- mouse Cystatin C antibody 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100)
  • Sample diluent buffer 30ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
Background Cystatin C or cystatin 3(formerly gamma trace, post-gamma-globulin or neuroendocrine basic polypeptide), a protein encoded by the CST3 gene, was originally described as a constituent of normal cerebrospinal fluid(CSF) and of urine from patients with renal failure. Cystatin 3 has a low molecular weight(approximately 13.3 kilodaltons), and it is removed from the bloodstream by glomerular filtration in the kidneys. In mouses, all cells with a nucleus(cell core containing the DNA) produce cystatin C as a chain of 120 amino acids. It is found in virtually all tissues and bodily fluids. Cystatin C, which belongs to the type II cystatin gene family, is a potent inhibitor of lysosomal proteinases(enzymes from a special subunit of the cell that break down proteins) and probably one of the most important extracellular inhibitors of cysteine proteases(it prevents the breakdown of proteins outside the cell by a specific type of protein degrading enzymes). Moreover, cystatin C is involved in network reorganization in the epileptic dentate gyrus.
Gene Symbol Cst3
Gene ID 13010
Other products for "Cst3"
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