Mouse Cathepsin D ELISA Kit

CAT#: EA100431

Mouse Cathepsin D ELISA Kit


 Product Datasheet for 'EA100431'

USD 506.00


Availability*
3 Weeks

Size
    • 96-Well

Product images

Specifications

Product Data
Description Mouse Cathepsin D ELISA Kit
Size 96-well
Format 8×12 divisible strips
Assay Type Sandwich ELISA kit of Quantitative Detection for Mouse Cathepsin D
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 156pg/ml-10,000pg/ml
Sample Type Cell culture supernates, serum and plasma(heparin, EDTA).
Sample Volume 100 ul
Specificity This kit is used for quantitative detection of mouse Cathepsin D
Sensitivity <10pg/ml
Reactivity Mouse
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Interference No significant interference observed with available related molecules.
Components
  • 96-well plate precoated with anti- mouse Cathepsin D antibody 1
  • Lyophilized recombinant mouse Cathepsin D standard 10ng/tubex2
  • Biotinylated anti- mouse Cathepsin D antibody 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100)
  • Sample diluent buffer 30 ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
Background Cathepsin D is a protein that in humans is encoded by the CTSD gene. This proteinase is a member of the peptidase C1 family, having a specificity similar to but narrower than that of pepsin A. It is mapped to 11p15.5. The cDNA encodes a 412-amino acid protein with 20 and 44 amino acids in a pre- and prosegment, respectively. Cathepsin D is one of the lysosomal proteinases. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease and it has been considered as a breast cancer tumor marker.
Gene Symbol Ctsd
Gene ID 13033
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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