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Mouse Cathepsin B ELISA Kit

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SKU
EA100429
Mouse Cathepsin B ELISA Kit
  $625.00
3 Weeks*
Specifications
Specifications
Product Data
Species Mouse
Assay Type Sandwich ELISA kit of Quantitative Detection for Mouse Cathepsin B
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 156pg/ml-10,000pg/ml
Sample Type Cell culture supernates, serum and plasma(heparin, EDTA).
Sample Volume 100 ul
Specificity This kit is used for quantitative detection of mouse Cathepsin B
Sensitivity <5pg/ml
Reactivity Mouse
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Interference No significant interference observed with available related molecules.
Components
  • 96-well plate precoated with anti-mouse Ctsb antibody 1
  • Lyophilized recombinant mouse Ctsb standard 10ng/tubex2
  • Biotinylated anti-mouse Ctsb antibody 100ul (dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 100ul (dilution 1:100)
  • Sample diluent buffer 30ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
  • Plate Sealers 4 x
  • Wash Buffer (25x) 20 ml
Background Cathepsin B is an enzymatic protein belonging to the peptidase or protease families. In humans, it is coded by the CTSB gene.1, 2 And this gene is mapped to chromosome 8p22.3 The protein encoded by this gene is a lysosomal cysteine proteinase composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. It is a member of the peptidase C1 family. Cathepsin B was once suspected as a candidate protease participating in the conversion of beta-amyloid precursor protein into the amyloid plaques found in Alzheimer's disease patients. However, this function is now known to be mediated by BACE1 protease. It is now thought that cathepsin B can degrade beta-amyloid precursor protein into harmless fragments. Thus, it is conceivable cathepsin B may play a pivotal role in the natural defense against Alzheimer's disease.4 Overexpression of cathepsin B has been associated with esophageal adenocarcinoma and other tumors. At least five transcript variants encoding the same protein have been found for this gene. The standard product used in this kit is recombinant mouse Cathepsin B with the molecular mass of 37KDa.
Gene Symbol Ctsb
Locus ID 13030
Shipping Blue Ice
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Documents
Resources

file-alt Citations

Development of NR0B2 as a therapeutic target for the re-education of tumor associated myeloid cells
Vidana Gamage, Albright, Smith et al
Cancer Lett (2024)
Cited by 13
AI Summary: OriGene’s Cathepsin B ELISA kit (EA100429) was used in this study to measure Cathepsin B levels, contributing to the evaluation of immune cell function in the development of NR0B2 as a therapeutic target for tumor-associated myeloid cells.
Snippet: IL-1β (432604) and Cathepsin B (EA100429) enzyme-linked immunosorbent assay (ELISA) kits were from BioLegend (San Diego, CA) and Origene (Rockville, MD) respectively. Fetal bovine serum (FBS) was from Cytiva HyClone. Non-essential amino acids, sodium pyruvate, penicillin/streptomycin, DPBS without Ca2+ and Mg2+, cell
NR0B2 re-educates myeloid immune cells to reduce regulatory T cell expansion and progression of breast and other solid tumors
Vidana Gamage, Shahoei, Wang et al
Cancer Lett (2024) 597, 217042 DOI: 10.1016/j.canlet.2024.217042
Cited by 8
AI Summary: OriGene’s Cathepsin B (EA100429) ELISA kit was used in this study to measure Cathepsin B levels, contributing to the investigation of how NR0B2 regulates myeloid cell function and tumor progression.
Snippet: Cathepsin B (EA100429) enzyme-linked immunosorbent assay (ELISA) kits were from BioLegend (San Diego, CA) and Origene (Rockville, MD) respectively. Fetal bovine serum (FBS) was from Cytiva HyClone. Non-essential amino acids, sodium pyruvate, penicillin/streptomycin, DPBS
Showing 1-2 of 2 papers.
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