Mouse BAFF ELISA Kit

CAT#: EA100424

Mouse BAFF ELISA Kit


 Product Datasheet for 'EA100424'

USD 535.00


Availability*
3 Weeks

Size
    • 96-Well

Product images

Specifications

Product Data
Description Mouse BAFF ELISA Kit
Size 96-well
Format 8×12 divisible strips
Assay Type Sandwich ELISA kit of Quantitative Detection for Mouse BAFF
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 46.9pg/ml-3000pg/ml
Sample Type Cell culture supernates, serum and plasma(heparin, EDTA).
Sample Volume 100 ul
Specificity This kit is used for quantitative detection of mouse BAFF
Sensitivity <10pg/ml
Reactivity Mouse
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Interference No significant interference observed with available related molecules.
Components
  • 96-well plate precoated with anti- mouse BAFF antibody 1
  • Lyophilized recombinant mouse BAFF standard 10ng/tubex2
  • Biotinylated anti- mouse BAFF antibody 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100)
  • Sample diluent buffer 30ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
Background B-cell activating factor(BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFLSF13B gene.1,2 BAFF is a cytokine that belongs to the tumor necrosis factor(TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. This cytokine is expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells.3All these ligands act as heterotrimers(i.e. three of the same molecule) interacting with heterotrimeric receptors4, although BAFF has been known to be active as either a hetero- or homotrimer.5 The standard used in this kit is recombinant mouse soluble BAFF(A127-L309) with molecular weight 23.2KDa.
Gene Symbol Tnfsf13b
Gene ID 24099

Documents

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