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Rat MMP-2 ELISA Kit

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SKU
EA100413
Rat MMP-2 ELISA Kit
  $755.00
3 Weeks*
Specifications
Specifications
Product Data
Species Rat
Assay Type Sandwich ELISA kit of Quantitative Detection for Rat MMP-2
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 156pg/ml-10,000pg/ml
Sample Type Cell culture supernates, serum and plasma(heparin).
Sample Volume 100 ul
Specificity This kit is used for quantitative detection of rat MMP-2
Sensitivity <10pg/ml
Reactivity Rat
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Interference No significant interference observed with available related molecules.
Components
  • 96-well plate precoated with anti- rat MMP-2 antibody 1
  • Lyophilized recombinant rat MMP-2 standard 10ng/tubex2
  • Biotinylated anti- rat MMP-2 antibody 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100)
  • Sample diluent buffer 30ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
Background Type IV collagenase, 72-kD, is officially designated matrix metalloproteinase-2(MMP2). It is also known as gelatinase, 72-kD. MMP-2 plays an essential role in angiogenesis and arteriogenesis, two processes critical to restoration of tissue perfusion after ischemia. MMP-2 expression is increased in tissue ischemia, but the responsible mechanisms remain unknown.1 Matrix metalloproteinases(MMPs) catalyze extracellular matrix degradation. Control of their activity is a promising target for therapy of diseases characterized by abnormal connective tissue turnover. MMPs are expressed as latent proenzymes that are activated by proteolytic cleavage that triggers a conformational change in the propeptide(cysteine switch). The structure of proMMP-2 reveals how the propeptide shields the catalytic cleft and that the cysteine switch may operate through cleavage of loops essential for propeptide stability.2 The gene is localized to 16q21 using somatic cell hybrids and in situ hybridization.3 The standard product used in this kit is recombinant rat MMP-2, consisting of 662 amino acids with the molecular mass of 72KDa. The detected MMP-2 includes zymogen and active enzyme.
Gene Symbol Mmp2
Locus ID 81686
Shipping Blue Ice
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Documents
Resources

file-alt Citations

Matrine Exerts a Strong Anti-Arthritic Effect on Type II Collagen-Induced Arthritis in Rats by Inhibiting Inflammatory Responses
Pu, Fang, Li et al
Int J Mol Sci (2016) 17 (9) DOI: 10.3390/ijms17091410
Cited by 65
AI Summary: OriGene's rat MMP-2 ELISA kit (cat. No. EA100413) was used in this study to measure MMP-2 levels in serum, helping assess the anti-arthritic effects of matrine in collagen-induced arthritis rats.
Snippet: (Wuhan, China);rat IL-1β (cat. No. ab100768), rat IL-6 (cat. No. ab100772) and rat IL-17A (cat. No. ab119536) ELISA kits were purchased from the Abcam Co. (Cambridge, UK);rat MMP-2 ELISA kits (cat. No. EA100413) was purchased from OriGene Technologies, ... Cite SaveSaving
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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