Human VEGF-C ELISA Kit

CAT#: EA100404

Human VEGF-C ELISA Kit


 Product Datasheet for 'EA100404'

USD 542.00


Availability*
3 Weeks

Size
    • 96-Well

Product images

Specifications

Product Data
Description Human VEGF-C ELISA Kit
Size 96-well
Format 8×12 divisible strips
Assay Type Sandwich ELISA kit of Quantitative Detection for Human VEGF-C
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 62.5pg/ml-4000pg/ml
Sample Type Cell culture supernates, serum and plasma(heparin, EDTA).
Sample Volume 100 ul
Specificity This kit is used for quantitative detection of human VEGF-C
Sensitivity <3pg/ml
Reactivity Human
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Interference No significant interference observed with available related molecules.
Components
  • 96-well plate precoated with anti- human VEGF-C antibody 1
  • Lyophilized recombinant human VEGF-C standard 10ng/tubex2
  • Biotinylated anti- human VEGF-C antibody 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100)
  • Sample diluent buffer 30ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
Background Vascular endothelial growth factor C is a VEGF. The human gene encoding it is VEGFC. The protein encoded by this gene is a member of the platelet-derived growth factor/vascular endothelial growth factor(PDGF/VEGF) family, is active in angiogenesis, lymphangiogenesis and endothelial cell growth and survival, and can also affect the permeability of blood vessels. This secreted protein undergoes a complex proteolytic maturation, generating multiple processed forms which bind and activate VEGFR-3 receptors. Only the fully processed form can bind and activate VEGFR-2 receptors. This protein is structurally and functionally similar to vascular endothelial growth factor D(VEGF-D). The C terminus of VEGFC has cysteine-rich repeat units characteristic of the Balbiani ring 3 protein(BR3P) of the midge Chironomus tentans.1,2 The standard product used in this kit is recombinant human VEGF-C, consisting of 135 amino acids with the molecular mass of 23Kda after glycosylation.
Gene Symbol VEGFC
Gene ID 7424
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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