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Rat VEGF ELISA Kit

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SKU
EA100377
Rat VEGF ELISA Kit
  $755.00
3 Weeks*
Specifications
Specifications
Product Data
Species Rat
Assay Type Sandwich ELISA kit of Quantitative Detection for Rat VEGF
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 15.6pg/ml-1000pg/ml
Sample Type Cell culture supernates, serum and plasma(heparin, EDTA, citrate).
Sample Volume 100 ul
Specificity This kit is used for quantitative detection of rat VEGF
Sensitivity <1pg/ml
Reactivity Rat
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Interference No significant interference observed with available related molecules.
Components
  • 96-well plate precoated with anti- rat VEGF antibody 1
  • Lyophilized recombinant rat VEGF standard 10ng/tubex2
  • Biotinylated anti- rat VEGF antibody 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100)
  • Sample diluent buffer 30ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
Background Vascular permeability factor/vascular endothelial growth factor(VPF/VEGF), a potent cytokine expressed by most malignant tumors, has critical roles in vasculogenesis and both physiological and pathological angiogenesis. VEGF produced by tumor cells potently stimulates endothelial cell proliferation and angiogenesis and plays a key role in the pathophysiology of several neoplasias. VEGF may also play a pivotal role in mediating the development and progression of diabetic retinopathy. VEGF, a major regulator of angiogenesis, binds to two receptor tyrosine kinases, KDR/Flk-1 and Flt-1. The VEGF gene is mapped by fluorescence in situ hybridization to chromosome 6p12.The standard product used in this kit is recombinant rat VEGF164, which is a 25KDa single chain as well as a 50KDa dimer.
Gene Symbol Vegfa
Locus ID 83785
Shipping Blue Ice
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Documents
Resources

file-alt Citations

Diazoxide protects rat vascular endothelial cells against hypoxia and cold-induced damage
Zhang, Huang, Li et al
Exp Ther Med (2017) 13 (6), 3257-3266 DOI: 10.3892/etm.2017.4437
Cited by 6
AI Summary: OriGene products contributed to this study by providing the anti-VEGF antibody (cat. no. TA364117) used to assess vascular endothelial growth factor levels in rat vascular endothelial cells exposed to hypoxia and cold-induced damage.
FigureFigureFigure
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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