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Human VCAM-1 ELISA Kit

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SKU
EA100374
Human VCAM-1 ELISA Kit
  $755.00
3 Weeks*
Specifications
Specifications
Product Data
Species Human
Assay Type Sandwich ELISA kit of Quantitative Detection for Human VCAM-1
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 156pg/ml-10,000pg/ml
Sample Type Cell culture supernates, serum and plasma(heparin, EDTA).
Sample Volume 100 ul
Specificity This kit is used for quantitative detection of human VCAM-1
Sensitivity <10pg/ml
Reactivity Human
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Interference No significant interference observed with available related molecules.
Components
  • 96-well plate precoated with anti- human VCAM-1 antibody 1
  • Lyophilized recombinant human VCAM-1 standard 10ng/tubex2
  • Biotinylated anti- human VCAM-1 antibody 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100)
  • Sample diluent buffer 30ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
Background Vascular cell adhesion molecule 1(VCAM-1) is a cell surface glycoprotein adhesive for certain blood leukocytes and tumor cells, which is expressed by activated endothelium in a variety of pathologic conditions including atherosclerosis. Increased expression of VCAM1 is associated with a variety of chronic inflammatory conditions, making its expression and function a target for therapeutic intervention. Integrin alpha4beta1(VLA-4) and VCAM-1 facilitate a critical cell-cell adhesion event required for survival of endothelial and mural cells during vascularization. The VCAM1 gene is assigned to the 1p31-32 region of chromosome 1 based on the analysis of human-mouse hybrid cell lines and in situ hybridization. The standard product used in this kit is recombinant human VCAM-1, consisting of Phe25-Glu698 amino acid sequence of a single chain with the molecular mass of 74KDa. As a result of glycosylation, the molecular mass of 90-95KDa is revealed by SDS-PAGE.
Gene Symbol VCAM1
Locus ID 7412
Shipping Blue Ice
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Documents
Resources

file-alt Citations

Association between serum cell adhesion molecules with hs-CRP, uric acid and VEGF genetic polymorphisms in subjects with metabolic syndrome
Ghazizadeh, Rezaei, Avan et al
Mol. Biol. Rep. (2019) 47 (2), 867-875 DOI: 10.1007/s11033-019-05081-2
Cited by 24
AI Summary: OriGene's ELISA kits for E-selectin, ICAM-1, and VCAM-1 were used to measure serum cell adhesion molecule levels, enabling the study to assess their association with metabolic syndrome and VEGF genetic polymorphisms.
Snippet: Measurement of serum CAM levels. Serum levels of E-selectin, ICAM-1 and VCAM-1 were determined using commercial ELISA kits (IBL International GMBH and ORIGENE) and an Awareness/Stat Fax 2100 analyzer. Statistical analyses;- Measurement of serum CAM levels. Serum levels of E-selectin, ICAM-1 and VCAM-1 were determined using commercial ELISA kits (IBL International GMBH and ORIGENE) and an Awaren...
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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