Mouse TNF alpha ELISA Kit

CAT#: EA100367

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Mouse TNF alpha ELISA Kit



USD 534.00


Availability*
3 Weeks

Size
    • 1 x 96 wells

Product images

Specifications

Product Data
Assay Type Sandwich ELISA kit of Quantitative Detection for Mouse TNF alpha
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 15.6 pg/ml-1000 pg/ml
Sample Type Cell culture supernates, serum and plasma (heparin, EDTA).
Sample Volume 100 ul
Specificity This kit is used for quantitative detection of mouse TNF alpha
Sensitivity <1pg/ml
Reactivities Mouse
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Interference No significant interference observed with available related molecules.
Components
  • 96-well plate precoated with anti-mouse TNF alpha antibody | 1
  • Lyophilized recombinant mouse TNF alpha standard | 10ng/tube×2
  • Biotinylated anti-mouse TNF alpha antibody | 100μl (dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) | 100 ul (dilution 1:100)
  • Sample diluent buffer | 30ml
  • Antibody diluent buffer | 12ml
  • ABC diluent buffer | 12ml
  • TMB color developing agent | 10ml
  • TMB stop solution | 10ml
  • Plate Sealers | 4
  • Wash Buffer (25x) | 20 ml
Background Tumor necrosis factor-alpha(TNF-alpha, or TNF) is secreted by macrophages in response to inflammation, infection and cancer. Human Tumor Necrosis Factor(TNF) and Lymphotoxin(TNF-beta) are cytotoxic proteins which have similar biological activities and share 30% amino acid homology. TNF-alpha is produced by monocytes, which can stimulate endothelial cells to produce the multilineage growth factor granulocyte-macrophage colony-stimulating factor and extend the role of this immunoregulatory protein to the regulation of hematopoiesis in vitro. TNF is a soluble protein that causes damage to tumor cells but has no effect on normal cells. Human TNF has been purified to apparent homogeneity as a 17.3-kilodalton protein from HL-60 leukemia cells and has showed cytotoxic and cytostatic activities against various human tumor cell lines. The human TNF cDNA is 1585 base pairs in length and encodes a protein of 233 amino acids. The mature protein begins at residue 77, leaving a long leader sequence of 76 amino acids. TNF-alpha has been mapped to human chromosome 6.
Gene Symbol Tnf
Gene ID 21926
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.