Mouse TNF alpha ELISA Kit

SKU EA100367

Mouse TNF alpha ELISA Kit

Product Data

• Species: Mouse
• Assay Type: Sandwich ELISA kit of Quantitative Detection for Mouse TNF alpha
• Assay Length: 3.5 hours incubations; 1 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 15.6 pg/ml-1000 pg/ml
• Sample Type: Cell culture supernates, serum and plasma (heparin, EDTA).
• Sample Volume: 100 ul
• Specificity: This kit is used for quantitative detection of mouse TNF alpha
• Sensitivity: <1pg/ml
• Reactivity: Mouse
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Interference: No significant interference observed with available related molecules.
• Components:
  • 96-well plate precoated with anti-mouse TNF alpha antibody | 1
  • Lyophilized recombinant mouse TNF alpha standard | 10ng/tube×2
  • Biotinylated anti-mouse TNF alpha antibody | 100μl (dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) | 100 ul (dilution 1:100)
  • Sample diluent buffer | 30ml
  • Antibody diluent buffer | 12ml
  • ABC diluent buffer | 12ml
  • TMB color developing agent | 10ml
  • TMB stop solution | 10ml
  • Plate Sealers | 4
  • Wash Buffer (25x) | 20 ml
• Background: Tumor necrosis factor-alpha(TNF-alpha, or TNF) is secreted by macrophages in response to inflammation, infection and cancer. Human Tumor Necrosis Factor(TNF) and Lymphotoxin(TNF-beta) are cytotoxic proteins which have similar biological activities and share 30% amino acid homology. TNF-alpha is produced by monocytes, which can stimulate endothelial cells to produce the multilineage growth factor granulocyte-macrophage colony-stimulating factor and extend the role of this immunoregulatory protein to the regulation of hematopoiesis in vitro. TNF is a soluble protein that causes damage to tumor cells but has no effect on normal cells. Human TNF has been purified to apparent homogeneity as a 17.3-kilodalton protein from HL-60 leukemia cells and has showed cytotoxic and cytostatic activities against various human tumor cell lines. The human TNF cDNA is 1585 base pairs in length and encodes a protein of 233 amino acids. The mature protein begins at residue 77, leaving a long leader sequence of 76 amino acids. TNF-alpha has been mapped to human chromosome 6.
• Gene Symbol: Tnf
• Locus ID: 21926
• Shipping: Blue Ice