Rat TNF alpha ELISA Kit

CAT#: EA100366

Rat TNF alpha ELISA Kit

Specifications

Product Data

• Assay Type: Sandwich ELISA kit of Quantitative Detection for Rat TNF alpha
• Assay Length: 3.5 hours incubations; 1 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 15.6 pg/ml - 1000 pg/ml
• Sample Type: Cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
• Sample Volume: 100 ul
• Specificity: This kit is used for quantitative detection of rat TNF alpha
• Sensitivity: <1pg/ml
• Reactivities: Rat
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Interference: No significant interference observed with available related molecules.
• Components:
  • 96-well plate precoated with anti- rat TNF alpha antibody | 1
  • Lyophilized recombinant rat TNF alpha standard 10ng/tube | 2
  • Biotinylated anti- rat TNF alpha antibody 100ul (dilution 1:100) | 1
  • Avidin-Biotin-Peroxidase Complex(ABC) 100ul (dilution 1:100) | 1
  • Sample diluent buffer 30ml | 1
  • Antibody diluent buffer 12ml | 1
  • ABC diluent buffer 12ml | 1
  • TMB color developing agent 10ml | 1
  • TMB stop solution 10ml | 1
  • Plate Sealers | 4
  • Wash Buffer (25x) 20 ml | 1
• Background: Tumor necrosis factor-alpha(TNF-alpha, or TNF) is secreted by macrophages in response to inflammation, infection and cancer. Human Tumor Necrosis Factor(TNF) and Lymphotoxin(TNF-beta) are cytotoxic proteins which have similar biological activities and share 30% amino acid homology. TNF-alpha is produced by monocytes, which can stimulate endothelial cells to produce the multilineage growth factor granulocyte-macrophage colony-stimulating factor and extend the role of this immunoregulatory protein to the regulation of hematopoiesis in vitro. TNF is a soluble protein that causes damage to tumor cells but has no effect on normal cells. Human TNF has been purified to apparent homogeneity as a 17.3-kilodalton protein from HL-60 leukemia cells and has showed cytotoxic and cytostatic activities against various human tumor cell lines. The human TNF cDNA is 1585 base pairs in length and encodes a protein of 233 amino acids. The mature protein begins at residue 77, leaving a long leader sequence of 76 amino acids. TNF-alpha has been mapped to human chromosome 6. The standard product used in this kit is recombinant rat TNFalpha, consisting of 157 amino acids with the molecular mass of 17KDa.
• Gene Symbol: Tnf
• Gene ID: 24835