Human TNF alpha ELISA Kit
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Assay Type | Sandwich ELISA kit of Quantitative Detection for Human TNF alpha |
| Assay Length | 3.5 hours incubations; 1 hour washing and analyzing samples |
| Signal | Colorimetric |
| Curve Range | 15.6pg/ml-1000pg/ml |
| Sample Type | Cell culture supernates, serum and plasma (heparin, EDTA, citrate). |
| Sample Volume | 100 ul |
| Specificity | This kit is used for quantitative detection of human TNF alpha |
| Sensitivity | <1pg/ml |
| Reactivity | Human |
| Cross Reactivity | There is no detectable cross-reactivity with other relevant proteins. |
| Interference | No significant interference observed with available related molecules. |
| Components |
|
| Background | Tumor necrosis factor-alpha(TNF-alpha, or TNF) is secreted by macrophages in response to inflammation, infection and cancer. Human Tumor Necrosis Factor(TNF) and Lymphotoxin(TNF-beta) are cytotoxic proteins which have similar biological activities and share 30% amino acid homology. TNF-alpha is produced by monocytes, which can stimulate endothelial cells to produce the multilineage growth factor granulocyte-macrophage colony-stimulating factor and extend the role of this immunoregulatory protein to the regulation of hematopoiesis in vitro. TNF is a soluble protein that causes damage to tumor cells but has no effect on normal cells. Human TNF has been purified to apparent homogeneity as a 17.3-kilodalton protein from HL-60 leukemia cells and has showed cytotoxic and cytostatic activities against various human tumor cell lines. The human TNF cDNA is 1585 base pairs in length and encodes a protein of 233 amino acids. The mature protein begins at residue 77, leaving a long leader sequence of 76 amino acids. TNF-alpha has been mapped to human chromosome 6. |
| Gene Symbol | TNF |
| Locus ID | 7124 |
| Shipping | Blue Ice |
Reviews
Documents
| Datasheet | |
| SDS | |
Citations
Sex hormone-dependent and -independent regulation of serum BAFF and TNF in cohorts of transgender and cisgender men and women
Tsatsanis, Elenkov, Leijonhufvud et al
Endocr Connect (2023) 12 (3) DOI: 10.1530/EC-22-0456
Tsatsanis, Elenkov, Leijonhufvud et al
Endocr Connect (2023) 12 (3) DOI: 10.1530/EC-22-0456
AI Summary: In this study, OriGene’s TNF ELISA kit (Cat No EA100365) was used to quantitatively measure serum TNF levels, enabling the authors to assess sex hormone-dependent and -independent regulation of TNF among transgender and cisgender individuals.
Snippet: ol/L. BAFF (lowest detection level 1.01 pg/mL;CV 6.5% at 433 pg/mL) and TNF (lowest detection level 1 pg/mL;CV 5.5% at 93 pg/mL) were measured by ELISA (purchased from R&D Systems for BAFF and from OriGene for TNF, Cat No EA100365). All measured specimens gave values within the detection range with the exception of basal TNF levels in some samples, being below the lowest detection limit of the a
Resveratrol Mitigates Diabetic Testicular Dysfunction, Endocrine Deficits, and Insulin Resistance via Suppression of Sperm-Endocrine Aberrations and Oxidative Inflammation in Rats
Elsawy, Famurewa, Sedky
Andrologia (2023) 2023, 1-10 DOI: 10.1155/2023/6385767
Elsawy, Famurewa, Sedky
Andrologia (2023) 2023, 1-10 DOI: 10.1155/2023/6385767
Cited by 18
AI Summary: OriGene’s rat standard-ELISA kits (TNF-α Cat No: EA100365) were used in this study to measure testicular inflammatory cytokine levels, helping assess the effects of resveratrol on oxidative inflammation in diabetic rats.
Snippet: The testicular levels of TNF-α (Cat No: EA100365), IL-4 (Cat No: MBS162452), IL-10 (Cat No: MBS764911), and IL-6 (Cat No: MBS726707) were measured with rat standard-ELISA kits procured from OriGene Technologies Inc., Rockville, MD and MyBioSource, Inc., San Diego, USA, following the manufacturers’ directions
Synthesis, Anticancer, Antioxidant, Anti-Inflammatory, Antimicrobial Activities, Molecular Docking, and DFT Studies of Sultams Derived from Saccharin
Al-Fayez, Elsawy, Mansour et al
Molecules (2022) 27 (20) DOI: 10.3390/molecules27207104
Al-Fayez, Elsawy, Mansour et al
Molecules (2022) 27 (20) DOI: 10.3390/molecules27207104
Cited by 7
AI Summary: OriGene’s Human TNFα ELISA Kit (Cat. No. EA100365) was used in this study to measure the anti-inflammatory activity of the synthesized sultam compounds by quantifying TNF-α levels.
Snippet: dried to give crude esters (3c–g). 3.4. In Vivo Assay 3.4.1. Materials CCl4 (Cat. No. 56-23-5) was purchased from Loba Chemie (India), Human TNFα ELISA Kit (Cat. No. EA100365) was purchased from OriGene Technologies Inc., Rockville, MD, USA, Rat Interleukin 6 (IL-6) ELSA kit (Cat. No. MBS726707) was obtained from MyBioSource, Inc., San Diego, CA, USA;total antioxidant capacity kit (Cat. No
In vivo evaluation of the protective effects of arjunolic acid against lipopolysaccharide-induced septic myocardial injury
Elsawy, Almalki, Elmenshawy et al
PeerJ (2022) 10, e12986 DOI: 10.7717/peerj.12986
Elsawy, Almalki, Elmenshawy et al
PeerJ (2022) 10, e12986 DOI: 10.7717/peerj.12986
Cited by 13
AI Summary: In this study, OriGene’s Human TNF alpha ELISA Kit (Cat. No. EA100365) was used to measure tumor necrosis factor-alpha (TNF-α) levels, contributing to the assessment of inflammatory responses in evaluating the protective effects of arjunolic acid against LPS-induced septic myocardial injury.
Snippet: EK0978;Bosten Scientific, Marlborough, MA, USA) according to the manufacturer’s protocol. Tumor necrosis factor-alpha (TNF- α) was estimated using Human TNF alpha ELISA Kit (Cat. No. EA100365;l OriGene Technologies Inc., Rockville, MD, USA). Interleukin 1 (IL-1), interleukin 4 (IL-4) and interleukin 10 (IL-10) were determined using standard ELISA methods with commercialized kits (IL-1;Cat
Apigenin Ameliorates Lead Acetate induced Hyperlipidemia, Hypothyroidism and Hypogonadism in Male Rats
Algefare, Sedky, Alfwuaires et al
Research Square (2021)
Algefare, Sedky, Alfwuaires et al
Research Square (2021)
Cited by 2
Immunization with neural-derived peptides increases neurogenesis in rats with chronic spinal cord injury
Rodríguez-Barrera, Flores-Romero, García et al
CNS Neurosci Ther (2020) 26 (6), 650-658 DOI: 10.1111/cns.13368
Rodríguez-Barrera, Flores-Romero, García et al
CNS Neurosci Ther (2020) 26 (6), 650-658 DOI: 10.1111/cns.13368
Cited by 18
AI Summary: In this study, the OriGene TNF-α ELISA Kit was used to measure TNF-α protein levels, helping to assess the inflammatory response in rats following immunization with neural-derived peptides after chronic spinal cord injury.
Snippet: Eight weeks after PBS or A91 immunization, animals were euthanized with an overdose of sodium pentobarbital (80 mg/kg) and the SC samples were rapidly excised. Reagents, samples, and standards were prepared according to the instructions of rat IL‐4 ELISA Kit (Cell Applications), IL‐10 ELISA Kit (RayBiotech), BDNF ELISA Kit (Ray Biotech), GAP‐43 ELISA Kit (Cusabio), and TNF‐α ELISA Kit (OriGene). …
Showing 1-6 of 6 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
Related Products
- Price:
- Actual Price:
Welcome to OriGene AI!
I'm your AI-powered guide to everything OriGene.
Ask me anything:
- Product specs
- Ordering help
- Technical questions
- How to find exactly what your experiment needs
Prefer a live agent? Connect with us by clicking the green chat icon, as seen in the image below or email our team at custsupport@origene.com.
