Human PLGF ELISA Kit

CAT#: EA100342

Human PLGF ELISA Kit

Datasheet
SDS

USD 705.00

2 Weeks*

Size
    • 1 x 96 wells

Product Images

Specifications

Product Data
Assay Type Sandwich ELISA kit of Quantitative Detection for Human Placental Growth Factor
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 15.6pg/ml-1000pg/ml
Sample Type Cell culture supernates, serum, plasma(heparin, EDTA, citrate) and urine.
Sample Volume 100 ul
Specificity This kit is used for quantitative detection of human PLGF
Sensitivity <1pg/ml
Reactivities Human
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Interference No significant interference observed with available related molecules.
Components
  • 96-well plate precoated with anti-human PLGF antibody | 1
  • Lyophilized recombinant human PLGF standard | 10ng/tube x 2
  • Biotinylated anti-human PLGF antibody | 100ul (dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) | 100ul (dilution 1:100)
  • Sample diluent buffer | 30ml
  • Antibody diluent buffer | 12ml
  • ABC diluent buffer | 12ml
  • TMB color developing agent | 10ml
  • TMB stop solution | 10ml
  • Plate Sealers | 4
  • Wash Buffer (25x) | 20 ml
Background The protein, also called PGF, is 149 amino acids long and shares 53% identity with the platelet-derived growth factor-like region of human VPF. And the N-glycosylated PLGF protein is secreted into the medium and that it functions as a dimer1. The PLGF gene is mapped to 14q24-q31. There are 3 isoforms of PGF, designated PGF1, PGF2, and PGF3. Only PGF2 is able to bind heparin. Additionally, PGF regulates inter- and intramolecular cross-talk between the VEGF receptor tyrosine kinases FLT1 and FLK12. It also can stimulate angiogenesis and collateral growth in ischemic heart and limb with at least a comparable efficiency to VEGF3.
Gene Symbol PGF
Gene ID 5228
Standard Curve
Representative standard curve for Human PLGF PicoKine ELISA Kit

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.