Human OPG ELISA Kit

CAT#: EA100335

Human OPG ELISA Kit

Product Images

Representative standard curve for Human OPG PicoKine ELISA Kit

Specifications

Product Data

• Assay Type: Sandwich ELISA kit of Quantitative Detection for Human OPG
• Assay Length: 3.5 hours incubations; 1 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 93.7pg/ml-6000pg/ml
• Sample Type: Cell culture supernates, serum, plasma(heparin, EDTA), saliva and urine.
• Sample Volume: 100 ul
• Specificity: This kit is used for quantitative detection of human OPG
• Sensitivity: <5pg/ml
• Reactivities: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Interference: No significant interference observed with available related molecules.
• Components:
  • 96-well plate precoated with anti-human OPG antibody | 1
  • Lyophilized recombinant human OPG standard | 10ng/tubex2
  • Biotinylated anti-human OPG antibody | 100ul (dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC) | 100ul (dilution 1:100)
  • Sample diluent buffer | 30ml
  • Antibody diluent buffer | 12ml
  • ABC diluent buffer | 12ml
  • TMB color developing agent | 10ml
  • TMB stop solution | 10ml
  • Adhesive cover | 4
  • Wash Buffer (25x) | 20ml
• Background: Osteoprotegerin(OPG) is identical to osteoclastogenesis inhibitory factor(OCIF), a soluble member of the tumor-necrosis factor receptor family that inhibits osteoclastogenesis. OPG is considered to play an important role in the regulation of bone resorption by modifying osteoclast differentiation. Osteoprotegerin is an independent risk factor for the progression of atherosclerosis and onset of cardiovascular disease. It can act as a soluble factor in the regulation of bone mass and imply a utility for OPG in the treatment of osteoporosis associated with increased osteoclast activity. OPG system may play a critical role in the development of osteolytic bone disease in multiple myeloma and that targeting this system may have therapeutic potential. OPG also plays a role in the vascular system. The standard product used in this kit is recombinant OPG, a 55KDa glycoprotein.
• Gene Symbol: TNFRSF11B
• Gene ID: 4982
• Standard Curve:
  
  Representative standard curve for Human OPG PicoKine ELISA Kit