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Human MMP13 ELISA Kit

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SKU
EA100326
Human MMP13 ELISA Kit
  $755.00
3 Weeks*
Specifications
Specifications
Product Data
Species Human
Assay Type Sandwich ELISA kit of Quantitative Detection for Human MMP13
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 156 pg/ml- 10.000pg/ml
Sample Type Cell culture supernates, serum and plasma(heparin).
Sample Volume 100 ul
Specificity This kit is used for quantitative detection of human MMP13
Sensitivity < 5pg/ml
Reactivity Human
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Interference No significant interference observed with available related molecules.
Components
  • 96-well plate precoated with anti-human MMP13 antibody | 1
  • Lyophilized recombinant human MMP13 standard | 10 ng/tube x 2
  • Biotinylated anti-human MMP13 antibody | 100 ul (dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) | 100 ul (dilution 1:100)
  • Sample diluent buffer | 30 ml
  • Antibody diluent buffer | 12 ml
  • ABC diluent buffer | 12 ml
  • TMB color developing agent | 10 ml
  • TMB stop solution | 10 ml
  • Adhesive cover | 4
  • Wash Buffer (25x) | 20 ml
Background Collagenase 3 is an enzyme that in humans is encoded by the MMP13 gene. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The protein encoded by this gene cleaves type II collagen more efficiently than types I and III. The expression of MMP13 in osteoarthritic cartilage and its activity against type II collagen indicates that the enzyme plays a significant role in cartilage collagen degradation and must, therefore, form part of a complex target for proposed therapeutic interventions based on collagenase inhibition. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3.
Gene Symbol MMP13
Locus ID 4322
Standard Curve
Shipping Blue Ice
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Documents
Resources

file-alt Citations

Evaluation of biomarkers of joint damage in patients subjected to arthroscopy
Izaguirre, González-Gutiérrez, Galindo-López et al
Int Orthop (2020)
Cited by 5
AI Summary: OriGene's Human MMP-13 ELISA kit was used in this study to measure MMP-13 levels in patient serum and synovial fluid samples, contributing to the evaluation of joint damage biomarkers in knee osteoarthritis.
Snippet: The production of Human MMP-13 (OriGene/ABCAMF ), Human MMP-3 (Invitrogen ), Human C-telopeptide of type I collagen (CTX-I, MyBioSource company ), Human Cross Linked C-telopeptide of type II collagen (CTX-II, MyBioSource company ), and Human
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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