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Human MMP-9 ELISA Kit

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SKU
EA100324
Human MMP-9 ELISA Kit
  $755.00
3 Weeks*
Specifications
Specifications
Product Data
Species Human
Assay Type Sandwich ELISA kit of Quantitative Detection for Human MMP-9
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 156pg/ml-10,000pg/ml
Sample Type Cell culture supernates, serum, plasma(heparin), saliva and urine.
Sample Volume 100 ul
Specificity This kit is used for quantitative detection of human MMP-9
Sensitivity <5pg/ml
Reactivity Human
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Interference No significant interference observed with available related molecules.
Components
  • 96-well plate precoated with anti- human MMP-9 antibody 1
  • Lyophilized recombinant human MMP-9 standard 10ng/tubex2
  • Biotinylated anti- human MMP-9 antibody 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100)
  • Sample diluent buffer 30ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
Background The 92-kD type IV collagenase is also known as 92-kD gelatinase, type V collagenase, gelatinase B, or matrix metalloproteinase-9(MMP9). The 72- and 92-kDa type IV collagenases are members of a group of secreted zinc metalloproteases. The matrix metalloproteinases(MMPs) are able to degrade the extracellular matrix and allow angiogenesis and tumor invasion. Gelatinase B, a matrix metalloproteinase that has proteolytic activity against connective tissue proteins, has been suggested to be important in the connective tissue remodeling processes associated with atherogenesis and plaque rupture. MMP-9 is predominantly expressed in neutrophils, macrophages, and mast cells, rather than in oncogene-positive neoplastic cells. The polymorphism of MMP-9 acts as a genetic factor for the development of smoking-induced pulmonary emphysema. The standard product used in this kit is recombinant human MMP-9 with the molecular mass of 95KDa. The detected MMP-9 includes zymogen and active enzyme.
Gene Symbol MMP9
Locus ID 4318
Standard Curve
Shipping Blue Ice
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Documents
Resources

file-alt Citations

The Alteration of Plasma Matrix Metalloproteinase-9 Level after the Addition of Bromelin 500 mg to Standard Therapy of Acute Ischemic Stroke and Its Correlation with Outcome
Sinurat, Sjahrir, Rambe et al
Open Access Maced J Med Sci (2018) 6 (4), 624-628 DOI: 10.3889/oamjms.2018.151
Snippet: Matrix metalloproteinase-9 concentrations in plasma samples were analysed using MMP9 human ELISA Assay Kit (EA 100106-Origene) according to manufacturers’ instructions and Chemwell 2910 analyser. Limit of detection of MMP9 human ELISA Assay Kit (EA 100106-Origene) is 31 pg/ml or 0.3 μg/ml
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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