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Mouse MMP-3 ELISA Kit

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SKU
EA100321
Mouse MMP-3 ELISA Kit
  $755.00
3 Weeks*
Specifications
Specifications
Product Data
Species Mouse
Assay Type Sandwich ELISA kit of Quantitative Detection for Mouse MMP-3
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 156pg/ml-10,000pg/ml
Sample Type Cell culture supernates, serum and plasma(heparin).
Sample Volume 100 ul
Specificity This kit is used for quantitative detection of mouse MMP-3
Sensitivity <10pg/ml
Reactivity Mouse
Cross Reactivity There is cross-reactivates with MMP-10 approximately 2% and no detectable cross-reactivity with other MMPs.
Interference No significant interference observed with available related molecules.
Components
  • 96-well plate precoated with anti- mouse MMP-3 antibody 1
  • Lyophilized recombinant mouse MMP-3 standard 10ng/tubex2
  • Biotinylated anti- mouse MMP-3 antibody 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100)
  • Sample diluent buffer 30ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
Background Matrix metalloproteinase-3(MMP-3) also called stromelysin or transin, is a proteoglycanase closely related to collagenase(MMP1) with a wide range of substrate specificities. The complete primary structure for human MMP-3, which has 477 residues including a 17-residue signal peptide. MMP-3 and collagenase are 54% identical in sequence, suggesting a common origin for the evolution of the two proteinases. MMP-3 and collagenase expression are coordinately modulated in synovial fibroblast cultures. MMP-3 is a secreted metalloprotease produced predominantly by connective tissue cells. Together with other metalloproteases, it can synergistically degrade the major components of the extracellular matrix. It is capable of degrading proteoglycan, fibronectin, laminin, and type IV collagen, but not interstitial type I collagen. MMP-3 genotype may be an important determinant of vascular remodeling and age-related arterial stiffening, with the heterozygote having the optimal balance between matrix accumulation and deposition.
Gene Symbol Mmp3
Locus ID 17392
Standard Curve
Shipping Blue Ice
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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