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Human IL-12(p70) ELISA Kit

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SKU
EA100287
Human IL-12(p70) ELISA Kit
  $625.00
3 Weeks*
Specifications
Specifications
Product Data
Species Human
Assay Type Sandwich ELISA kit of Quantitative Detection for Human IL-12(p70)
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 7.8pg/ml-500pg/ml
Sample Type Cell culture supernates and serum.
Sample Volume 100 ul
Specificity This kit is used for quantitative detection of human IL-12(p70)
Sensitivity <2pg/ml
Reactivity Human
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Interference No significant interference observed with available related molecules.
Components
  • 96-well plate precoated with anti- human IL-12(P70) antibody 1
  • Lyophilized recombinant human IL-12(P70) standard 10ng/tubex2
  • Biotinylated anti- human IL-12(P70) antibody 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100)
  • Sample diluent buffer 30ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
Background Interleukin(IL)-12 is a 70-KDa cytokine comprised of two disulfide-linked proteins(p35 and p40) and is essential for the initiation of effective immune response.i And the IL-12p70 is a heterodimer of p35 and p40 subunits; it is an important cytokine secreted by antigen-presenting cells in response to antigenic stimulation.ii Gene expression analysis of the IL-12 cytokine family subunits revealed that both strains induced high levels of p40(protein chain communal to IL-12 p70 and IL-23) as well as p19, a subunit of IL-23. Conversely only ACT- 18HS19 infection induced consistent transcription of IL-12 p35, a subunit of IL-12 p70.iii The standard product used in this kit is recombinant human IL-12 p70 with the molecular mass of 75 KDa.
Gene Symbol IL12A
Locus ID 3592
Standard Curve
Shipping Blue Ice
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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