Rat IL-6 ELISA Kit

CAT#: EA100281

Rat IL-6 ELISA Kit

Product Images

Representative standard curve for Rat IL-6 PicoKine ELISA Kit

Specifications

Product Data

• Assay Type: Sandwich ELISA kit of Quantitative Detection for Rat IL-6
• Assay Length: 3.5 hours incubations; 1 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 62.5 pg/ml-4000 pg/ml
• Sample Type: ell culture supernates, serum and plasma(heparin, EDTA, citrate).
• Sample Volume: 100 ul
• Specificity: This kit is used for quantitative detection of rat IL-6
• Sensitivity: < 5 pg/ml
• Reactivities: Rat
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Interference: No significant interference observed with available related molecules.
• Components:
  • 96-well plate precoated with anti- rat IL-6 antibody 1
  • Lyophilized recombinant rat IL-6 standard 10 ng/tube x 2
  • Biotinylated anti- rat IL-6 antibody 100 ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 100 ul(dilution 1:100)
  • Sample diluent buffer 30 ml
  • Antibody diluent buffer 12 ml
  • ABC diluent buffer 12 ml
  • TMB color developing agent 10 ml
  • TMB stop solution 10 ml
  • Plate seaeler 4 Piece
  • Wash Buffer (25x) 20 ml
  • 
    
• Background: Interleukin-6(IL-6) is a protein that in humans is encoded by the IL6 gene. Interleukin-6(IL-6) is involved not only in the hepatic acute phase response but also in adipose tissue metabolism, lipoprotein lipase activity, and hepatic triglyceride secretion. IL-6 is a pleiotropic cytokine that plays a critical role in bone resorption. IL-6 has been suggested to stimulate the HPA axis during immune activation independent of the input of hypothalamic corticotropin-releasing hormone(CRH). Interleukin-6(IL-6) is an immunoregulatory cytokine that activates a cell-surface signaling assembly composed of IL-6, the IL-6 alpha-receptor(IL-6Ralpha), and the shared signaling receptor gp130. The standard product used in this kit is recombinant rat IL-6, consisting of 188 amino acids with the molecular mass of 21.8kDa.
• Gene Symbol: Il6
• Gene ID: 24498
• Standard Curve:
  
  Representative standard curve for Rat IL-6 PicoKine ELISA Kit