Human IL-5 ELISA Kit

CAT#: EA100277

Human IL-5 ELISA Kit

USD 705.00

3 Weeks*

    • 1 x 96 wells

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Product Data
Assay Type Sandwich ELISA kit of Quantitative Detection for Human IL-5
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 7.8pg/ml-500pg/ml
Sample Type Cell culture supernates, serum, plasma(heparin, EDTA) and urine.
Sample Volume 100 ul
Specificity This kit is used for quantitative detection of human IL-5
Sensitivity <2pg/ml
Reactivities Human
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Interference No significant interference observed with available related molecules.
  • 96-well plate precoated with anti- human IL-5 antibody 1
  • Lyophilized recombinant human IL-5 standard 10ng/tubex2
  • Biotinylated anti- human IL-5 antibody 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100)
  • Sample diluent buffer 30ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
Background Interleukin-5(IL-5) is also known as eosinophil differentiation factor(EDF). IL-5 is a potential candidate gene in the pathogenesis of asthma, as it is the main cytokine controlling eosinophil activity and eosinophils are pivotal in the development of airway inflammation. The predicted amino acid sequence of 134 amino acids is identical with that recently reported for human interleukin-5 but shows no significant homology with other known hemopoietic growth regulators. IL-5 is a lineage-specific hematopoietic growth factor that stimulates the production of eosinophils and eosinophil colonies from normal human bone marrow cells. IL-5 gene to human chromosome 5 at bands q23-31. Human and mouse of IL-5 share 70% amino acid sequence homology.
Gene Symbol IL5
Gene ID 3567
Standard Curve
Representative standard curve for Human IL-5 PicoKine ELISA Kit


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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.