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Rat IL-1 beta ELISA Kit

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SKU
EA100264
Rat IL-1 beta ELISA Kit
  $625.00
2 Weeks*
Specifications
Specifications
Product Data
Species Rat
Assay Type Sandwich ELISA kit of Quantitative Detection for Rat IL-1 beta
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 31.2pg/ml-2000pg/ml
Sample Type Cell culture supernates, serum and plasma(heparin, EDTA).
Sample Volume 100 ul
Specificity This kit is used for quantitative detection of rat IL-1 beta
Sensitivity <1pg/ml
Reactivity Rat
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Interference No significant interference observed with available related molecules.
Components
  • 96-well plate precoated with anti-rat IL1B antibody | 1
  • Lyophilized recombinant rat IL1B standard | 10ng/tube x 2
  • Biotinylated anti-rat IL1B antibody | 100ul (dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) | 100ul (dilution 1:100)
  • Sample diluent buffer | 30ml
  • Antibody diluent buffer | 12ml
  • ABC diluent buffer | 12ml
  • TMB color developing agent | 10ml
  • TMB stop solution | 10ml
  • Plate Sealers 4 x
  • Wash Buffer (25x) 20 ml
Background The Interleukin 1 family is a group of 11 beta trefoil cytokines. Interleukin-1, produced mainly by blood monocytes, mediates the panoply of host reactions collectively known as acute phase response . Interleukin-1beta(IL-1beta) is a potent stimulator of bone resorption, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1beta belongs to proinflammatory cytokines, promote cancer cell adhesion and liver metastases by up-regulating the expression of vascular cell adhesion molecule-1(VCAM-1) on hepatic sinusoidal endothelium(HSE).
Gene Symbol Il1b
Locus ID 24494
Standard Curve
Shipping Blue Ice
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Documents
Resources

file-alt Citations

Corinthian Currants Supplementation Restores Serum Polar Phenolic Compounds, Reduces IL-1beta, and Exerts Beneficial Effects on Gut Microbiota in the Streptozotocin-Induced Type-1 Diabetic Rat
Kompoura, Prapa, Vasilakopoulou et al
Metabolites (2023) 13 (3) DOI: 10.3390/metabo13030415
Cited by 15
AI Summary: OriGene products, specifically the rat IL-1 beta ELISA kit (EA100264) and rat IL-6 ELISA kit (EA100168), were used in this study to measure inflammatory markers and assess the effects of Corinthian currant supplementation on inflammation in diabetic rats.
Snippet: y). Inflammatory markers, i.e., interleukin-1beta (IL-1b) and interleukin-6 (IL-6), were also determined by a sandwich ELISA method (rat IL-1 beta ELISA kit EA100264, and rat IL-6 ELISA kit EA100168, OriGene, Rockville, MD, USA) at the end of the intervention. 2.4.2. Determination of Polar Phenols in Rat Serum Polar Phenol Isolation from Serum Samples For the extraction of polar phenols, rat seru
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Showing 1-1 of 1 papers.
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