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IGFBP3

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SKU
EA100259
Mouse IGFBP-3 ELISA Kit
  $755.00
3 Weeks*
Specifications
Specifications
Product Data
Species Mouse
Assay Type Sandwich ELISA kit of Quantitative Detection for Mouse IGFBP-3
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 156pg/ml-10,000pg/ml
Sample Type Cell culture supernates and serum.
Sample Volume 100 ul
Specificity This kit is used for quantitative detection of mouse IGFBP-3
Sensitivity <10pg/ml
Reactivity Mouse
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Interference No significant interference observed with available related molecules.
Components
  • 96-well plate precoated with anti- mouse IGFBP-3 antibody 1
  • Lyophilized recombinant mouse IGFBP-3 standard 10ng/tubex2
  • Biotinylated anti- mouse IGFBP-3 antibody 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100)
  • Sample diluent buffer 30ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
Background Insulin-like growth factor(IGF)-binding protein-3(IGFBP-3) is a major determinant of circulating levels of the IGFs and is clinically useful for the evaluation of GH deficiency and for predicting the response to GH treatment. The circulating level of IGFBP-3 is inversely related to the risk of several common cancers, and that antiproliferative agents such as antiestrogens and retinoids act in part by up-regulating IGFBP-3 gene(IGFBP3) expression. Insulin-like growth factor-binding protein(IGFBP)-3, well characterized as the carrier of insulin-like growth factor(IGF), has been reported to have intrinsic bioactivity that is independent of IGF binding. IGFBP-3 has an IGF-independent, antiproliferative effect in undifferentiated and early differentiated but not in terminally differentiated chondrocytes. IGFBP-3 possesses both growth-inhibitory and potentiating effects on cells that are independent of IGF action and are mediated through specific. IGFBP-3 binding proteins/receptors locate at the cell membrane, cytosol, or nuclear compartments and in the extrIGFBP-3llular matrix. IGFBP3 is also located on chromosome 7. The standard product used in this kit is recombinant mouse IGFBP-3, consisting of 270 amino acids with the molecular mass of 29.4kDa. As a result of glycosylation, the molecular mass is 40-50KDa.
Gene Symbol IGFBP3
Locus ID 3486
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Documents
Resources

file-alt Citations

Functional modulation of IGF-binding protein-3 expression in melanoma
Dar, Majid, Nosrati et al
J Invest Dermatol (2010) 130 (8), 2071-9 DOI: 10.1038/jid.2010.70
Cited by 31
AI Summary: OriGene products contributed to this study by providing the human IGFBP3 cDNA (OriGene, SC119328) used for overexpression experiments, which enabled the authors to assess the effects of IGFBP3 on melanoma cell survival, apoptosis, and tumor growth.
FigureFigure
Showing 1-1 of 1 papers.
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