Human IGFBP2 ELISA Kit

SKU EA100256

Human IGFBP2 ELISA Kit

Product Data

• Species: Human
• Assay Type: Sandwich ELISA kit of Quantitative Detection for Human IGFBP2
• Assay Length: 3.5 hours incubations; 1 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 62.5pg/ml-4000pg/ml
• Sample Type: Cell culture supernates, serum, plasma(heparin, EDTA) and urine.
• Sample Volume: 100 ul
• Specificity: This kit is used for quantitative detection of human IGFBP2
• Sensitivity: <10pg/ml
• Reactivity: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Interference: No significant interference observed with available related molecules.
• Components:
  • 96-well plate precoated with anti- human IGFBP2 antibody 1
  • Lyophilized recombinant human IGFBP2 standard 10ng/tubex2
  • Biotinylated anti- human IGFBP2 antibody 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100)
  • Sample diluent buffer 30 ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
• Background: Insulin-like growth factor-binding protein 2(IGFBP2), also called IBP2, is a protein that in humans is encoded by the IGFBP2 gene. It is mapped to 2q35. By structural analysis, it showed that the IGFBP2 gene consists of 4 exons with 3 introns of lengths 27.0, 1.0, and 1.9 kb. IGFBP2 secreted by metastatic cells recruits endothelia by modulating IGF1 mediated activation of the IGF type-I receptor on endothelial cells. The IGFBP2/IGF1/IGF1R and GAS6/MERTK signaling pathways are regulators of cancer-mediated endothelial recruitment.
• Gene Symbol: IGFBP2
• Locus ID: 3485
• Shipping: Blue Ice