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Human ICAM-1 ELISA Kit

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SKU
EA100244
Human ICAM-1 ELISA Kit
  $625.00
3 Weeks*
Specifications
Specifications
Product Data
Species Human
Assay Type Sandwich ELISA kit of Quantitative Detection for Human ICAM-1
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 156pg/ml-10,000pg/ml
Sample Type Cell culture supernates, serum and plasma(heparin, EDTA).
Sample Volume 100 ul
Specificity This kit is used for quantitative detection of human ICAM-1
Sensitivity <10pg/ml
Reactivity Human
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Interference No significant interference observed with available related molecules.
Components
  • 96-well plate precoated with anti- human ICAM-1 antibody 1
  • Lyophilized recombinant human ICAM-1 standard 10ng/tubex2
  • Biotinylated anti- human ICAM-1 antibody 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100)
  • Sample diluent buffer 30ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
  • Plate Sealers 4 x
  • Wash Buffer 1 Powder Pack for 1000 ml
Background Intercellular adhesion molecule-1(ICAM-1) is an integral membrane protein, a member of the immunoglobulin superfamily, and a ligand for lymphocyte function-associated(LFA) antigens, a beta 2 leukocyte integrin. The normal function of human intercellular adhesion molecule-1(ICAM-1) is to provide adhesion between endothelial cells and leukocytes after injury or stress. ICAM-1 binds to leukocyte function-associated antigen(LFA-1) or macrophage-1 antigen(Mac-1). ICAM-1 is found on leukocytes, fibroblasts, epithelial cells and endothelial cells and its expression is regulated by inflammatory cytokines. ICAM-1 has a tissue distribution similar to that of the major histocompatibility complex class II antigens and is likely to play a role in inflammatory responses.1
Gene Symbol ICAM1
Locus ID 3383
Standard Curve
Shipping Blue Ice
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Documents
Resources

file-alt Citations

Association of the apoptotic markers Apo1/Fas and cCK-18 and the adhesion molecule ICAM-1 with Type 1 diabetes mellitus in children and adolescents
Kostopoulou, Katsa, Ioannidis et al
BMC Pediatr (2024) 24 (1), 493 DOI: 10.1186/s12887-024-04926-5
Cited by 2
AI Summary: OriGene’s ICAM-1 ELISA kit (EA100244) was used in this study to quantitatively measure ICAM-1 levels in participants, contributing to the assessment of its association with Type 1 diabetes mellitus in children and adolescents.
Snippet: sed. The MC30 ELISA was used to detect caspase-cleaved CK18 produced during the early stages of apoptosis, (VLVbio Hästholmsvägen, Sweden) and for ICAM-1, the Sandwich Elisa kit was used (ELISA kit OriGene Technologies, Inc. EA100244, Rockville, Maryland USA). For Apo1/Fas the intra-assay coefficient of variation (CVs) was 4.5% and the calculated inter-assay CV was 3.1%. For cCK-18 (M30) the in
Figure
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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