Human HGF ELISA Kit

SKU EA100243

Human HGF ELISA Kit

Product Data

• Species: Human
• Assay Type: Sandwich ELISA kit of Quantitative Detection for Human HGF
• Assay Length: 3.5 hours incubations; 1 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 125pg/ml-8000pg/ml
• Sample Type: Cell culture supernates, serum and plasma(EDTA, citrate).
• Sample Volume: 100 ul
• Specificity: This kit is used for quantitative detection of human HGF
• Sensitivity: <5pg/ml
• Reactivity: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Interference: No significant interference observed with available related molecules.
• Components:
  • 96-well plate precoated with anti- human HGF antibody 1
  • Lyophilized recombinant human HGF standard 10ng/tubex2
  • Biotinylated anti- human HGF antibody 100ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 100ul(dilution 1:100)
  • Sample diluent buffer 30ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
  • Plate Sealers 4 x
  • Wash Buffer (25x) 20 ml
• Background: Hepatocyte growth factor(HGF) is the most potent mitogen for mature parenchymal hepatocytes in primary culture, and seems to be a hepatotrophic factor that acts as a trigger for liver regeneration after partial hepatectomy and liver injury. HGF has a relative molecular mass(Mr) of 82,000 and is a heterodimer composed of a large alpha-subunit of Mr 69,000 and a small beta-subunit of Mr 34,000. The protein consists of 728 amino acid residues, including a possible signal peptide at the N-terminus. HGF may serve as a paracrine mediator to control placental development and growth. This growth factor may play an important role as a paracrine mediator of the proliferation of melanocytes and endothelial cells, as well as cells of epithelial origin. The gene encoding the human HGF is assigned to human chromosome 7.
• Gene Symbol: HGF
• Locus ID: 3082
• Shipping: Blue Ice