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Human Fibronectin ELISA Kit

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SKU
EA100226
Human Fibronectin ELISA Kit
  $755.00
2 Weeks*
Specifications
Specifications
Product Data
Species Human
Assay Type Sandwich ELISA kit of Quantitative Detection for Human Fibronectin
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 156pg/ml-10,000g/ml
Sample Type Cell culture supernates, serum and plasma(heparin, EDTA, citrate).
Sample Volume 100 ul
Specificity This kit is used for quantitative detection of human Fibronectin
Sensitivity <10pg/ml
Reactivity Human
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Interference No significant interference observed with available related molecules.
Components
  • 96-well plate precoated with anti- human Fibronectin antibody 1
  • Lyophilized natural human Fibronectin standard 10ng/tubex2
  • Biotinylated anti- human Fibronectin antibody 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100)
  • Sample diluent buffer 30ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
Background Fibronectin(FN) also known as LETS, is identified on the surfFN of fibroblasts by labeling with radioactive compounds or specific antibodies. Fibronectin is a 430,000-dalton dimeric glycoprotein that exists in 2 forms, termed cellular and plasma fibronectin. Cellular and plasma fibronectins are heterodimers consisting of similar but not identical polypeptides. These two forms of FN differ in biologic activity. Fibronectins bind cell surfFNs and various compounds including collagen, fibrin, heparin, DNA, and actin. Because fibronectin stimulates endocytosis in several systems and promotes the clearance of particulate material from the circulation, it could function in the clearance of C1q-coated material such as immune complexes or cellular debris. Fibronectins are involved in cell adhesion, cell motility, opsonization, would healing, and maintenance of cell shape. LETS, encoded on chromosome 8, is responsible for the LETS protein expression in humans. Because LETS has been implicated in tumorigenicity and cellular transformation, it is of interest that rearrangement or modifications in the number of chromosome 8 have been associated with certain forms of cancer. The standard used in this kit is isolated from human plasma with the molecular mass of 200-250KDa.
Gene Symbol FN1
Locus ID 2335
Standard Curve
Shipping Blue Ice
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Documents
Resources

file-alt Citations

Changes in serum fibronectin levels predict tumor recurrence in patients with early hepatocellular carcinoma after curative treatment
Kim, Cho, Lee et al
Sci Rep (2020) 10 (1), 21313 DOI: 10.1038/s41598-020-78440-w
Cited by 19
AI Summary: OriGene's commercial fibronectin measurement kits were used to quantify serum fibronectin levels in patient samples, enabling the study to assess the association between fibronectin changes and hepatocellular carcinoma recurrence.
Snippet: Overnight-fasting serum samples were obtained from all patients before and after 4-6 weeks of initial treatment, and stored at − 80 °C. Serum fibronectin was measured using standard commercial kits (Origene, Rockville, MD, USA) by a researcher who was blind to
Figure
Cytokine Receptor Profiling in Human Colonic Subepithelial Myofibroblasts: A Differential Effect of Th Polarization-Associated Cytokines in Intestinal Fibrosis
Filidou, Valatas, Drygiannakis et al
Inflamm. Bowel Dis. (2018)
AI Summary: OriGene’s Human Fibronectin Enzyme-Linked Immunosorbent Assay (ELISA) kit was used to measure fibronectin levels in colonic subepithelial myofibroblast supernatants, enabling the assessment of cytokine effects on fibronectin expression in this study.
Snippet: Human Fibronectin Enzyme-Linked Immunosorbent Assay Human fibronectin was measured in cSEMF superna- tants according to the manufacturer's instructions (Human Fibronectin Enzyme-Linked Immunosorbent Assay [ELISA] kit, Origene, Rockville, MD, USA)
Showing 1-2 of 2 papers.
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