Mouse Eotaxin ELISA Kit

SKU EA100218

Mouse Eotaxin ELISA Kit

Product Data

• Species: Mouse
• Assay Type: Sandwich ELISA kit of Quantitative Detection for Mouse Eotaxin
• Assay Length: 3.5 hours incubations; 1 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 15.6pg/ml-1000pg/ml
• Sample Type: Cell culture supernates, serum and plasma(citrate, EDTA).
• Sample Volume: 100 ul
• Specificity: This kit is used for quantitative detection of mouse Eotaxin
• Sensitivity: <1pg/ml
• Reactivity: Mouse
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Interference: No significant interference observed with available related molecules.
• Components:
  • 96-well plate precoated with anti- mouse Eotaxin antibody 1
  • Lyophilized recombinant mouse Eotaxin standard 10ng/tubex2
  • Biotinylated anti- mouse Eotaxin antibody 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100)
  • Sample diluent buffer 30ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
• Background: Eotaxin, also known as CCL11, is a potent inducer of eosinophil chemotaxis and is considered as a selective ligand of the CC chemokine receptor 3(CCR3), which is expressed on eosinophils, basophils, and Th2 lymphocytes. Eotaxin is assumed to be involved in eosinophilic inflammatory diseases such as atopic dermatitis, allergic rhinitis, asthma and parasitic infections. The gene maps to chromosome 17 and is expressed constitutively at high levels in small intestine and colon, and at lower levels in various other tissues. The deduced mature protein sequence is 66% identical to human monocyte chemoattractant protein-1, and 60% identical to guinea pig eotaxin.
• Gene Symbol: Ccl11
• Locus ID: 20292
• Shipping: Blue Ice