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Mouse BDNF ELISA Kit

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SKU
EA100207
Mouse BDNF ELISA Kit
  $625.00
3 Weeks*
Specifications
Specifications
Product Data
Species Mouse
Assay Type Sandwich ELISA kit of Quantitative Detection for Mouse BDNF
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 31.2pg/ml-2000pg/ml
Sample Type Cell culture supernates, serum and plasma(heparin, EDTA, citrate).
Sample Volume 100 ul
Specificity This kit is used for quantitative detection of mouse BDNF
Sensitivity <2pg/ml
Reactivity Mouse
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Interference No significant interference observed with available related molecules.
Components
  • 96-well plate precoated with anti- mouse BDNF antibody 1
  • Lyophilized recombinant mouse BDNF standard 10ng/tubex2
  • Biotinylated anti- mouse BDNF antibody 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100)
  • Sample diluent buffer 30ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
Background Brain-derived neurotrophic factor(BDNF) is a prosurvival factor induced by cortical neurons that is necessary for survival of striatal neurons in the brain. It is a secreted protein with the molecular weight of 27.8kDa, consisting of 247 amino acids. It is known to promote neuronal survival and differentiation. BDNF shares substantial amino acid sequence identity with nerve growth factor(NGF). BDNF and neurotrophin-3(NT-3) are two recently cloned neurotrophic factors that are homologous to NGF. mRNA products of the BDNF and NT-3 genes are detected in the adult human brain, suggesting that these proteins are involved in the maintenance of the adult nervous system. BDNF and other neurotrophins are critically involved in long-term potentiation(LTP). BDNF-mediated LTP is induced postsynaptically. BDNF has trophic effects on serotonergic(5-HT) neurons in the central nervous system. BDNF has an essential maintenance function in the regulation of anxiety-related behavior and in food intake through central mediators in both the basal and fasted state. It plays a role in treating breathing disorders such as respiratory insufficiency after spinal injury. The mature form of BDNF is identical in all mammals examined, and the gene encoding human BDNF to chromosome 11, band p13.
Gene Symbol Bdnf
Locus ID 12064
Standard Curve
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Documents
Resources

file-alt Citations

Selective targeting of the oligodendroglial GPR17 receptor improves myelin integrity and motor function in female SOD1G93A mice
Raffaele, Bonifacino, Mannella et al
bioRxiv (2026)
AI Summary: OriGene’s BDNF ELISA kit (cat. No. EA100207) was used in this study to quantify brain-derived neurotrophic factor (BDNF) levels in culture supernatants, enabling assessment of neurotrophic support related to myelin integrity and motor function.
Snippet: To assess brain-derived neurotrophic factor (BDNF) 177 release, before fixation culture supernatants were collected, centrifuged to remove debris, and 178 stored at −80 °C until analysis. BDNF levels were quantified using a commercially available 179 ELISA kit (cat. No. EA100207, OriGene Technologies, Herford, Germany), following the 180 manufacturer’s instruction
RAM-589.555 favors neuroprotective and anti-inflammatory profile of CNS-resident glial cells in acute relapse EAE affected mice
Zilkha-Falb, Rachutin-Zalogin, Cleaver et al
J Neuroinflammation (2020) 17 (1), 313 DOI: 10.1186/s12974-020-01983-2
Cited by 7
AI Summary: OriGene’s Mouse GDNF ELISA Kit (EA100590) was used in this study to measure glial cell-derived neurotrophic factor (GDNF) levels, contributing to the assessment of neurotrophic factor secretion by CNS-resident glial cells after RAM-589.555 treatment.
Snippet: or basic (FGF2) were measured by the enzyme-linked immunosorbent assay (ELISA;Quantikine ELISA total BDNF;DBNT00 and FGF basic;MFB00;R&D systems, Abingdon, UK and mouse GDNF ELISA Kits;EA100590;OriGene, Rockville, MD, USA, respectively) according to the manufacturer’s instructions. Samples were tested in duplicate and the mean was calculated. The intra- and interassay coefficients of varia
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Showing 1-2 of 2 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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