TMB Substrate, soluble, 1-part (100ml)

Snippet: was carried out by high-pressure heating. The slides were incubated with anti-CXCL14 antibody (1:200, Lot Ab137541;Abcam, Cambridge, UK) overnight at 4°C. …

Snippet: China, 1:100) were added to the slices and incubated in a humidified chamber at 4 °C overnight. For the next, the slices were incubated with the HRP*Polyclonal Goat Anti-Rabbit IgG (H + L) (PV-9001, Origene, Beijing, China) at room temperature for 20 min, followed by treatment with DAB substrate solution (ZLI-9019, Origene, Beijing, China). Finally, the slices were incubated with hematoxylin to

Snippet: immunohistochemistry (iHc) kits and reagents were purchased from ZSGB-Bio (cat. no. SP-9001;oriGene Technologies, Inc., Beijing, China). Dulbecco's cat. no. SP‑9001;ZSGB‑BIO;OriGene Technologies, Inc.) at 37˚C for 15 min;Immunohistochemistry (IHC) kits and reagents were purchased from ZSGB‑BIO (cat. no. SP‑9001;OriGene Technologies, Inc., Beijing, China). Dulbecco's cat. no. SP‑9001

Snippet: Primary antibodies against TF and ALB with corresponding biotin-coupled secondary antibodies were available from Abcam (Cambridge, MA, USA). 3,3′,5,5′-tetramethylbenzidine dihydrochloride (TMB) and hydrogen peroxide (H2O2) were purchased from Sigma-Aldrich (St. Louis, MO, USA). SA-HRP conjugate to oxidize TMB was obtained from Origene (Rockville, MD, USA). A mixture of 1:1 TMB and H2O2 substrate s...