Assay Buffer A for ELISA

Snippet: Briefly, plasma samples were diluted 1:16 in assay buffer A (AR100012, Origene) and pre-incubated with either 100 µg/ml of emicizumab or 20 IU/dl of FL-FVIII, and untreated samples analysed in parallel. Detection antibodies included PE-conjugated goat anti-human IgG Fc (12-4998-82

Snippet: TissueScan Real-Time PCR panels (HMRT100, 103, CSRT502) with Human β-actin control primer set (Origene) were used to determine the endogenous expression levels of CCM2 isoforms among the different tissues at the transcriptional level;- TissueScan Real-Time PCR panels (HMRT100, 103, CSRT502) with Human β-actin control primer set (Origene) were used to determine the endogenous expression levels of CCM2 isoforms among the different tissues at the transcriptional level;- TissueScan Real‑Time PCR panels (HMRT100, 103, CSRT502) with Human β‑actin control primer set (Origene) were used to determine the endogenous expression levels of CCM2 isoforms among the different tissues at the transcrip‑ tional level

Snippet: Radioimmunoprecipitation assay lysis and extrac‑ tion buffer, horseradish peroxidase‑conjugated AffiniPure goat anti‑mouse immunoglobulin (Ig)G and anti‑rabbit IgG antibodies and D‑glucose were purchased from OriGene Technologies, Inc., (Beijing, China)