Mouse monoclonal mGFP/mCFP/mYFP/KillerRed antibody, HRP conjugated

Snippet: y), p-p38 (#9211, Cell Signaling Technology, Boston, USA) and GAPDH (AP0063, Bioworld) were used. Subsequently, the membranes were incubated with HRP-conjugated secondary antibodies (1:8000, ZB-2301, Origene Technologies) for 1 h at room temperature. Protein bands were visualized with electrochemiluminescence substrate solution (GE Healthcare Life Sciences) and imaged by Micro chemiluminescence sy

Snippet: Following primary incubation, the membranes were incu‑ bated with a HRP conjugated secondary antibody (ZB‑2305;1:10,000;OriGene Technologies, Inc.) at 25˚C for 1 h. Protein bands were visualized using an ECL immunoreactive western blot detection reagent;- Following primary incubation, the membranes were incu‑ bated with a HRP conjugated secondary antibody (ZB‑2305

Snippet: C for overnight. The membrane was treated with HRP-conjugated secondary antibody (OriGene Technologies, Rockville, MD, USA) in ProNATM phospho-block solution to detect protein expression. The immunoreactive bands Cached

Snippet: We also used an HRP-conjugated secondary antibody (1:2000, ZSGB-Bio Origene, Beijing, China). All assays were performed in triplicate. Cell Proliferation Assay. The Cell Counting Kit-8 (CCK-8, Dojindo, Japan) was used to determine cell proliferation