Mouse monoclonal tdTomato antibody, clone OTI2H2

Snippet: anti-mouse-PD-L1 (ab213480;Abcam), anti-human/mouse CD274 (PD-L1, B7-H1) monoclonal antibody (MIH1;eBioscience;14-5983-82;Invitrogen), mouse monoclonal tdTomato antibody, clone OTI2H2 (TA180009;Origene), anti-human CD104 (Integrin β4) monoclonal antibody (439-9B) eFluor 660 eBioscience (50-1049-82;Invitrogen), anti-mouse-Integrin β4 antibody (EPR17517;ab182120;Abcam), anti-human integri;mouse monoclonal tdTomato antibody, clone OTI2H2 (TA180009;Abcam)

Snippet: excitation and emission wavelengths of 554 and 581 nm, respectively, for the detection of tdTomato. The fusion protein concentration was calculated against a dilution series of tdTomato standards (OriGene Technologies, Rockville, MD, USA) in the cell culture medium, taking into account the different molecular masses. Downstream processing and activity testing The culture broth or permeate was

Snippet: 1x TBS buffer for one time. Co-IP products were then analyzed by western blotting with antibodies against GFP (dilution factor, 1:3000;www.rokland-inc.com) and tdTomato (dilution factor, 1:2000;www.origene.com). For analysis of protein expression levels, protein extract was prepared by the NaOH lysis method43. Exponential cells were collected from 10 ml culture. After washed 1 time with 1 m

Snippet: lig2 (Peprotech;1:300 dilution), GFAP (Sigma-Aldrich and Abcam;1:250 dilution, respectively), Iba1 (Wako;1:200 dilution), CD68 (Bio-Rad;1:200 dilution), CD163 (Bio-Rad;1:200 dilution), tdTomato (Origene;1:500 dilution), SOX2 (R&D systems;1:300 dilution), TBR1 (Abcam;1:100 dilution), cleaved caspase-3 (Cell Signaling Technology;1:100 dilution), and Ki67 (Invitrogen;1:200 dilution). After