Anti-DDK (FLAG) mouse monoclonal antibody, clone F-tag-01
$405.00
2 Weeks*
Specifications
| Product Data | |
| Clone Name | F-tag-01 |
|---|---|
| Application | IF, WB |
| Recommended Dilution | WB: 1:1000, IP: 1:1000, IF: 1:1000 |
| Reactivity | DDK (FLAG) tag |
| Antibody Host | Mouse |
| Clonality | Monoclonal |
| Immunogen | A synthetic peptide (DYKDDDDK) coupled to KLH. |
| Isotype | IgG2b |
| Formulation | PBS (pH7.4) with 0.05% sodium azide |
| Concentration | lot specific |
| Conjugation | Unconjugated |
| Storage | Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap. Aliquot to avoid repeated freezing and thawing. |
| Stability | Stable for 1 year at -20°C from date of shipment. |
| Shipping | Blue Ice |
| Synonyms | ANTI DDK; ANTI FLAG; DDK; FLAG; FLAG TAG |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Citations
Ca+2 and Nε-lysine acetylation regulate the CALR-ATG9A interaction in the lumen of the endoplasmic reticulum
Braun, Sheehan, Shapiro et al
Sci Rep (2024) 14 (1), 25532 DOI: 10.1038/s41598-024-76854-4
Braun, Sheehan, Shapiro et al
Sci Rep (2024) 14 (1), 25532 DOI: 10.1038/s41598-024-76854-4
Cited by 1
AI Summary: OriGene products contributed to this study by providing primary antibodies (DDK, TA100011; tGFP, TA150071) and expression plasmids (pCMV6-SERCA-DDK, RC207626; pCMV6-ITPR3-DDK, RC222737; pCMV6-ATG9A-DDK, RC222513; pCMV6-ATG9A-tGFP, RG222568; pCMV6-CALR-DDK, RC203222) that were essential for detecting and expressing key proteins involved in investigating the regulation of ATG9A-CALR interactions in the endoplasmic reticulum.
Snippet: The following primary antibodies were used in this study: DDK (Origene, TA100011, 1:1000), ITPR3 (abcam, ab264283, 1:2000), tGFP (Origene, TA150071, 1:1000), and;itrogen, 2773051) and MEFs were transiently transfected with Lipofectamine STEM (Invitrogen, 01108889), according to manufacturer’s recommendations. Commercial plasmids used: human pCMV6-SERCA-DDK (Origene, RC207626), human pCMV6-ITPR3-DDK (Origene, RC222737), human pCMV6-ATG9A-DDK (Origene, RC222513), human pCMV6-ATG9A-tGFP (Origene, RG222568), human pCMV6-CALR-DDK (Origene, RC203222), ER signa
Mining cancer genomes for change-of-metabolic-function mutations
Tu, Diplas, Regal et al
Commun Biol (2023) 6 (1), 1143 DOI: 10.1038/s42003-023-05475-w
Tu, Diplas, Regal et al
Commun Biol (2023) 6 (1), 1143 DOI: 10.1038/s42003-023-05475-w
Cited by 2
AI Summary: OriGene's anti-FLAG antibody (TA100011) was used in immunoblotting experiments to detect exogenously expressed mutant proteins in the study.
Snippet: t 100 °C for 5 min, centrifuged 10 min 13,000 rpm at 4 °C and the supernatants were loaded onto a 4–12% Tris-glycine gel. Immunoblots were carried out using anti-FLAG (1:1000, TA100011, OriGene) as described previously40. To optimize immunoblots for membrane-associated proteins in SLC17A5-expressing cell samples, the same procedure was carried out without a boiling step. Instead, the
Insulin induction of SREBP-1c in rodent liver requires LXRα-C/EBPβ complex
Tian, Goldstein, Brown
PNAS (2016) 113 (29), 8182-7 DOI: 10.1073/pnas.1608987113
Tian, Goldstein, Brown
PNAS (2016) 113 (29), 8182-7 DOI: 10.1073/pnas.1608987113
Cited by 78
Showing 1-3 of 3 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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