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Carrier-free (BSA/glycerol-free) Mouse monoclonal eCFP antibody, clone OTI8A6

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SKU
CF180069
Carrier-free (BSA/glycerol-free) Mouse monoclonal eCFP antibody, clone OTI8A6
  $620.00
3 Days*
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Specifications
Specifications
Product Data
Clone Name OTI8A6 (formerly 8A6)
Application WB
Recommended Dilution WB: 1:2000
Reactivity eCFP
Antibody Host Mouse
Clonality Monoclonal
Immunogen This antibody is raised against a recombinant protein of eCFP expressed in HEK293 cells.
Isotype IgG2a
Formulation Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Conjugation Unconjugated
Storage Store at -20°C.
Stability Stable for 1 year at -20°C from date of shipment.
Shipping Ambient
Predicted Protein Size 26.9 kDa
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Other "OTI8A6 (formerly 8A6)" antibodies (5)
SKU Description Size Price
TA180069 Mouse monoclonal eCFP antibody, clone OTI8A6 100 ul
$447.00
TA180069S Mouse monoclonal eCFP antibody, clone OTI8A6 30 ul
$200.00
TA183013 Mouse monoclonal eCFP antibody, HRP conjugated 100 ul
$447.00
TA183036 Mouse Monoclonal eCFP Antibody, Magnetic Beads 1 ml
$776.00
TA183036S Mouse Monoclonal eCFP Antibody, Magnetic Beads 500 ul
$470.00

file-alt Citations

The θ-defensin retrocyclin 101 inhibits TLR4- and TLR2-dependent signaling and protects mice against influenza infection
Prantner, Shirey, Lai et al
J. Leukoc. Biol. (2017)
AI Summary: OriGene’s anti-eCFP monoclonal antibody (clone OTI8A6) was used in this study to visualize tagged TLR4 proteins by Western blot analysis, aiding in the investigation of how retrocyclin 101 inhibits TLR4- and TLR2-dependent signaling during influenza infection.
Snippet: www.jleukbio.org Page 3. were purchased from the indicated vendors. Mouse mAb were used to visualize TLR4 proteins with the tags FLAG (M2 clone) (Sigma-Aldrich) and eCFP (clone OTI8A6) (OriGene Technologies, Rockville, MD, USA) by Western blot analysis ... All 2 versions Cite SaveSaving
WB
Differential adapter recruitment by TLR2 co-receptors
Piao, Ru, Toshchakov
Pathog Dis (2016) 74 (5) DOI: 10.1093/femspd/ftw043
Cited by 26
AI Summary: OriGene’s mouse anti-eCFP antibody (8A6) was used for immunoprecipitation experiments to detect protein interactions involved in TLR2 signaling pathways in this study.
Snippet: A total of 100 μg protein of cell lysates were incubated with or without peptides (20 μM) for 1 h at 4°C, followed by 3 h or overnight incubation with 0.5 μg of mouse anti-eCFP antibody (8A6) (Origene, Rockville, MD) or anti-Flag M2 antibody (Sigma-Aldrich, St. ... Cite SaveSaving
IP
CD14 dependence of TLR4 endocytosis and TRIF signaling displays ligand specificity and is dissociable in endotoxin tolerance
Rajaiah, Perkins, Ireland et al
PNAS (2015) 112 (27), 8391-6 DOI: 10.1073/pnas.1424980112
Cited by 150
AI Summary: OriGene’s anti-eCFP monoclonal antibody was used for immunoprecipitation experiments to study TLR4 signaling pathways in this paper.
Snippet: …Lysates were precleared with protein G slurry (Roche) for 30 min at 4 °C. Lysates were subsequently immunoprecipitated for 3 h at 4 °C using 1 μg of anti-eCFP monoclonal antibody (Origene) with protein G slurry. …
IP WB
Recruitment of TLR adapter TRIF to TLR4 signaling complex is mediated by the second helical region of TRIF TIR domain
Piao, Ru, Piepenbrink et al
Proc Natl Acad Sci U S A (2013) 110 (47), 19036-41 DOI: 10.1073/pnas.1313575110
Cited by 59
AI Summary: OriGene products, specifically the mouse MyD88 knockout (SKU: KN-1523) and TRIF knockout (SKU: KN-1527) cell lines, were used in this study to assess the specificity and mechanism of action of TRIF-derived peptides in inhibiting TLR4-mediated signaling.
Showing 1-4 of 4 papers.
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