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GFP Goat Polyclonal Antibody

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SKU
AB0066-200
Goat Polyclonal Anti-GFP Antibody
Size:
600 ug 1.5 mg
Conjugation:
Unconjugated
  $485.00
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Specifications
Specifications
Product Data
Application IF, WB
Recommended Dilution WB 1:500-1:5,000; IF 1:50-1:1,000; IHC (paraffin) 1:50-1:1,000; IHC (frozen) 1:50-1:1,000
Reactivity GFP tag
Antibody Host Goat
Clonality Polyclonal
Immunogen Purified recombinant peptide produced in E. coli.
Isotype IgG
Formulation Polyclonal antibody supplied as a 200 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide.
Concentration 3mg/ml
Purification epitope affinity purified
Conjugation Unconjugated
Shipping Blue Ice
Background Green Fluorescent Protein
Note In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody does not recognize mCherry fluorescent protein.
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Documents
Resources

file-alt Citations

Affimer proteins are versatile and renewable affinity reagents
Tiede, Bedford, Heseltine et al
Elife (2017) 6 DOI: 10.7554/eLife.24903
AI Summary: In this paper, OriGene products such as the pCMV6-Entry expression vector were used to clone and express target proteins, facilitating the selection and characterization of Affimer proteins as versatile affinity reagents.
FigureFigureFigure
Bone marrow transplantation improves proximal tubule dysfunction in a mouse model of Dent disease
Gabriel, Belge, Gassama et al
Kidney Int (2017) 91 (4), 842-855 DOI: 10.1016/j.kint.2016.11.016
Cited by 39
AI Summary: In this study, OriGene products including the Mouse Clcn5 (NM_013491) qPCR primer pair (MP200990) and Mouse GAPDH (NM_008084) qPCR primer pair (MP200132) were used to measure gene expression levels, helping to demonstrate that wild-type bone marrow transplantation restores Clcn5 expression and improves proximal tubule function in a mouse model of Dent disease.
Reversible optogenetic control of kinase activity during differentiation and embryonic development
Krishnamurthy, Khamo, Mei et al
Development (2016) 143 (21), 4085-4094 DOI: 10.1242/dev.140889
Cited by 59
AI Summary: OriGene products contributed to this study by providing the pCMV6-Entry vector, which was used for cloning and expression of optogenetic constructs in mammalian cells.
Assessment of the expression and role of the α1-nAChR subunit in efferent cholinergic function during the development of the mammalian cochlea
Roux, Wu, McIntosh et al
J Neurophysiol (2016) 116 (2), 479-92 DOI: 10.1152/jn.01038.2015
Cited by 20
AI Summary: In this study, OriGene’s α1-nAChR cDNA (Cat# MR209316) and α1-nAChR shRNA plasmids (Cat# TL502473) were used to assess the expression and functional role of the α1-nAChR subunit in developing cochlear hair cells.
Identification of regions within the Legionella pneumophila VipA effector protein involved in actin binding and polymerization and in interference with eukaryotic organelle trafficking
Bugalhão, Mota, Franco
Microbiologyopen (2016) 5 (1), 118-33 DOI: 10.1002/mbo3.316
Cited by 18
AI Summary: OriGene products, specifically the anti-Myc antibody (TA150045) and anti-β-actin antibody (TA-09), were used in this study to detect the expression and analyze the actin-binding properties of VipA mutant proteins, thereby facilitating the investigation of VipA regions involved in actin interaction and organelle trafficking interference.
FigureFigureFigure
Evidence for hypothalamic ketone body sensing: impact on food intake and peripheral metabolic responses in mice
Carneiro, Geller, Fioramonti et al
Am J Physiol Endocrinol Metab (2016) 310 (2), E103-15 DOI: 10.1152/ajpendo.00282.2015
Cited by 43
AI Summary: OriGene products contributed to this paper by providing specific primers (OriGene, sequences in Table 1) used for quantitative PCR analysis of hypothalamic neuropeptide gene expression.
Showing 1-6 of 8 papers.
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