Rabbit F(ab')2 Anti-Sheep IgG (H&L) Antibody Phycoerythrin Conjugated, 500 ug
Specifications
| Product Data | |
| Application | FC, IF |
|---|---|
| Recommended Dilution | IF: 1:100 - 1:250 FC: 1:100 - 1:250 |
| Reactivity | Sheep |
| Antibody Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Sheep IgG whole molecule |
| Specificity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Rabbit Serum, Sheep IgG and Sheep Serum. No reaction was observed against anti-Pepsin or anti-Rabbit IgG F(c). |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free Preservative: 0.01% (w/v) Sodium Azide |
| Reconstitution Method | Restore with deionized water (or equivalent) - Reconstitution Volume: 1.0 mL |
| Concentration | 0.5 mg/mL - lot specific |
| Conjugation | RPE |
| Storage | Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis. |
| Shipping | Ambient |
| Note | F(ab')2 Anti-Sheep IgG Phycoerythrin Conjugated Secondary Antibody reagents are ideal for ELISA, western blotting, Immunohistochemistry, as well as other antibody detection methods. |
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