Goat F(ab')2 Anti-Rat IgG F(ab')2 Antibody Peroxidase Conjugated (Min X Bv Hs & Hu Serum Proteins), 500 uL

SKU
TA398216
Goat F(ab')2 Anti-Rat IgG F(ab')2 Antibody Peroxidase Conjugated (Min X Bv Hs & Hu Serum Proteins), 500 uL
  $545.00
5 Days*
Bulk/Customize
Specifications
Specifications
Product Data
Application ELISA, IHC, WB
Recommended Dilution WB: 1:5,000 - 1:200,000
IHC: 1:500 - 1:5,000
ELISA: 1:5,000 - 1:100,000
Reactivity Rat
Antibody Host Goat
Clonality Polyclonal
Immunogen Anti-Rat IgG F(ab')2 fragment was produced by repeated immunization with Rat IgG F(ab')2 fragment in goat.
Specificity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rat IgG, Rat IgG F(ab’)2 and Rat Serum.  No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(c) or Bovine, Horse and Human Serum Proteins.
Buffer 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Stabilizer: 15 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: None
Reconstitution Method Restore with deionized water (or equivalent) - Reconstitution Volume: 500 µL
Concentration 0.8 mg/mL - lot specific
Conjugation HRP
Storage Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
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Note Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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