Goat F(ab')2 Anti-Rabbit IgG (H&L) Antibody Peroxidase Conjugated, 500 ug
Specifications
| Product Data | |
| Application | ELISA, IHC, WB |
|---|---|
| Recommended Dilution | WB: 1:2,000 - 1:10,000 IHC: 1:500 - 1:2,500 ELISA: 1:100,000 |
| Reactivity | Rabbit |
| Antibody Host | Goat |
| Clonality | Polyclonal |
| Immunogen | Rabbit IgG whole molecule |
| Specificity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). Limited reactivity will occur against Armenian Hamster IgG. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! |
| Reconstitution Method | Restore with deionized water (or equivalent) - Reconstitution Volume: 500 µL |
| Concentration | 1.0 mg/mL - lot specific |
| Conjugation | HRP |
| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Shipping | Ambient |
| Note | F(ab')2 Anti-Rabbit IgG Peroxidase Conjugated Antibody has been tested by ELISA and is ideal for ELISA, western blotting, Immunohistochemistry, as well as other antibody detection methods. |
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