Goat Anti-Rat IgG (H&L) Antibody ATTO 532 Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rb & Sh Serum Proteins), 100 ug
Specifications
| Product Data | |
| Application | FLISA, IF, WB |
|---|---|
| Recommended Dilution | WB: >1:10,000 IF: >1:5,000 FLISA: >1:20,000 |
| Reactivity | Rat |
| Antibody Host | Goat |
| Clonality | Polyclonal |
| Immunogen | Rat IgG whole molecule |
| Specificity | Rat IgG (H&L) Antibody ATTO 532 was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG and Rat Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins. This antibody will react with heavy chains of rat IgG and with light chains of most rat immunoglobulins. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free Preservative: 0.01% (w/v) Sodium Azide |
| Reconstitution Method | Restore with deionized water (or equivalent) - Reconstitution Volume: 100 µL |
| Concentration | 1.0 mg/mL - lot specific |
| Conjugation | Unconjugated |
| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Shipping | Ambient |
| Note | Anti-Rat IgG (H&L) conjugated to ATTO 532 has been tested by dot blot and is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation. |
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