Rabbit Anti-Mouse IgG (gamma 1, 2a, 2b and 3 chain) Antibody ATTO 647N Conjugated, 100 ug
Specifications
| Product Data | |
| Application | FLISA, IF, WB |
|---|---|
| Recommended Dilution | WB: >1:10,000 IF: >1:5,000 FLISA: >1:20,000 |
| Reactivity | Mouse |
| Antibody Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | highly purified mouse IgG gamma 1, gamma 2a, gamma 2b and gamma 3 proteins |
| Specificity | Mouse IgG (gamma 1, 2a, 2b and 3 chain) Antibody ATTO 647N was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. This product shows balanced reactivity to Mouse IgG1, IgG2a, IgG2b and IgG3 proteins and is suitable to screen IgG class hybridoma clones. Minimal cross reactivity is observed against other Mouse immunoglobulin classes or light chain proteins. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free Preservative: 0.01% (w/v) Sodium Azide |
| Reconstitution Method | Restore with deionized water (or equivalent) - Reconstitution Volume: 100 µL |
| Concentration | 1.0 mg/mL - lot specific |
| Conjugation | Unconjugated |
| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Shipping | Ambient |
| Note | Anti-Mouse IgG (gamma 1, 2a, 2b and 3 chain) conjugated to ATTO 647N has been tested by dot blot and western blot and is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation. |
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