Goat Anti-Mouse IgG3 (Gamma 3 chain) Antibody Fluorescein Conjugated (Min X Bv, Hu, and Rb Serum Proteins), 1 mg
Specifications
| Product Data | |
| Application | FC, FLISA, IF, IHC, WB |
|---|---|
| Recommended Dilution | WB: 1:2,000 - 1:10,000 IHC: 1:1,000 - 1:5,000 IF: 1:1,000 - 1:5,000 FC: 1:200 - 1:500 FLISA: 1:10,000 - 1:50,000 |
| Reactivity | Mouse |
| Antibody Host | Goat |
| Clonality | Polyclonal |
| Immunogen | Mouse IgG3 heavy chain |
| Specificity | Anti-MOUSE IgG3 (Gamma 3 chain) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Goat Serum, Mouse Serum and Mouse IgG3. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1% of target signal. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free Preservative: 0.01% (w/v) Sodium Azide |
| Reconstitution Method | Restore with deionized water (or equivalent) - Reconstitution Volume: 1.0 mL |
| Concentration | 1.0 mg/mL - lot specific |
| Conjugation | FITC |
| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Shipping | Ambient |
| Note | Anti-Mouse IgG3 Fluorescein antibody has been tested by dot blot and is suitable for Flow Cytometry, Immunohistochemistry and Immunofluorescence Microscopy, ELISA, western blotting as well as other anti IgG2b antibody based assays. Mouse IgG3 secondary antibody is available in a variety of formats. |
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